AD样大鼠海马NMDAR亚单位2B和2A的表达及其与细胞凋亡的关系

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (2): 14-18.

AD样大鼠海马NMDAR亚单位2B和2A的表达及其与细胞凋亡的关系

刘志安1, 2，赵娓娓3，吕春娥2，李梅2，徐铁军1, 2

1. 江苏省脑病生物信息重点实验室，江苏徐州 221002；
2. 徐州医学院解剖和神经生物学教研室，江苏徐州 221002；
3. 第四军医大学附属西京医院放射科，西安 710032

收稿日期:2010-11-02 出版日期:2011-02-10 发布日期:2011-02-10
通讯作者: 徐铁军（1952- ），男，教授，博士，主要从事中枢神经损伤、再生和修复的分子机制和信号转导的研究。E-mail: xztjxu@163.com
作者简介:刘志安（1970- ），男，副教授，博士研究生，主要从事中枢神经退行性疾病的基础和应用的研究。E-mail: zhianliu@126.com
基金资助:
江苏省脑病生物信息重点实验室开放课题资助项目（JSBL0903）；江苏省高校研究生科技创新计划资助项目［苏教研（2009）10号］；江苏省高校自然科学基金资助项目（07KJB310117）；徐州医学院研究生科技创新计划资助项目［徐医研教(2010)6号］。

Expressions of NMDA receptor subunits NR2B and NR2A and their relationship with apoptosis in the hippocampus of Alzheimer′s diseaselike rats

LIU Zhi-an1, 2, ZHAO Wei-wei3, L Chun-e2, LI Mei2, XU Tie-jun1, 2

1. Jiangsu Province Key Laboratory of Brain Disease Bioinformation, Xuzhou 221002, Jiangsu, China;
2. Department of Anatomy and Neurobiology, Xuzhou Medical College, Xuzhou 221002, Jiangsu, China;
3. Department of Radiology, Affiliated Xijing Hospital of The Fourth Military Medical University, Xi′an 710032, China

Received:2010-11-02 Online:2011-02-10 Published:2011-02-10

摘要/Abstract

摘要：

目的研究N-甲基-D-天门冬氨酸受体(NMDAR) 亚单位2A和2B在阿尔茨海默病（AD）样大鼠海马的表达及其与细胞凋亡的关系。方法以Aβ1-40和AlCl3双干预方法建立AD样大鼠模型。采用免疫组化法检测NMDAR2B及NMDAR2A在AD样大鼠海马的表达，采用TUNEL法检测AD样大鼠海马细胞凋亡状况，并探讨二者的关系。结果在AD样大鼠海马各区NMDAR2B表达均显著增高（P﹤0.05）,CA1区最高，NMDAR2A未见显著变化（P>0.05），凋亡阳性细胞均显著增多，CA1区最多，其次为齿状回和CA3区（P﹤0.05）。结论 NMDAR2B在AD样大鼠海马表现为病理性高表达且与细胞凋亡以及选择性易损伤现象之间很可能存在着某种密切的关系。

关键词: 阿尔茨海默病；受体，N-甲基-D-天门冬氨酸；细胞凋亡；海马；大鼠

Abstract:

Objective To explore expressions of N-methyl-D-aspartate receptor subunits 2B (NR2B) and 2A (NR2A) and their relationship with apoptosis in the hippocampus of Alzheimer′s disease (AD)-like rats. Methods The AD-like rat model was established by intracerebroventricularly injecting Aβ1-40 and intraperitoneally injecting AlCl3. Expressions of NR2B and NR2A in the AD-like rat hippocampus were detected by immunohistochemistry, and the apoptosis in the AD-like rat hippocampus was detected by TUNEL staining. Then the relationship was further explored between them. Results Expression of NR2B was significantly increased in the AD-like rat hippocampus(P﹤0.05), especially in the CA1 subfield. Expression of NR2A had no significant change in the AD-like rat hippocampus(P>0.05). Apoptotic cells were significantly increased in the AD-like rat hippocampus (P﹤0.05), especially in the CA1 subfield, followed by the dentate gyrus and the CA3 subfield. Conclusion NR2B, not NR2A, has pathological high expression in the AD-like rat hippocampus. This pathological high expression may have a specific relationship with AD-like apoptosis and the selective vulnerable phenomenon of the rat hippocampus.

Key words: Alzheimer disease; Receptors, N-methyl-D-aspartate; Apoptosis; Hippocampus; Rats

中图分类号:

R322.81

刘志安1, 2，赵娓娓3，吕春娥2，李梅2，徐铁军1, 2. AD样大鼠海马NMDAR亚单位2B和2A的表达及其与细胞凋亡的关系[J]. 山东大学学报(医学版), 2011, 49(2): 14-18.

LIU Zhi-an1, 2, ZHAO Wei-wei3, L Chun-e2, LI Mei2, XU Tie-jun1, 2. Expressions of NMDA receptor subunits NR2B and NR2A and their relationship with apoptosis in the hippocampus of Alzheimer′s diseaselike rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(2): 14-18.

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