白藜芦醇对外周血单个核细胞中IL-1β、IL-6、MCP-1及SOCS1/3 表达的影响

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (11): 64-.

白藜芦醇对外周血单个核细胞中IL-1β、IL-6、MCP-1及SOCS1/3 表达的影响

彭洁1，韩雪莹2，胡晓燕3，邓景惕3，郭媛1

1. 山东大学齐鲁医院心血管内科，济南 250012； 2.山东省血液中心济南血站，济南 250001；
3. 山东大学医学院生物化学与分子生物学研究所，济南 250012

收稿日期:2011-05-17 出版日期:2011-11-10 发布日期:2011-11-10
通讯作者: 郭媛（1957- ），女，教授，硕士生导师，主要从事心血管疾病研究。 E-mail: guoyuan-cn@163.com
作者简介:彭洁（1985- ），女，硕士研究生，主要从事心血管疾病研究。

The effect of resveratrol on expression of IL-1β, IL-6, MCP-1 andSOCS1/3 in human peripheral blood mononuclear cells

PENG Jie1, HAN Xue-ying2, HU Xiao-yan3, DENG Jing-ti3, GUO Yuan1

1. Department of Cardiology, Qilu Hospital of Shandong University, Jinan 250012, China;
2. Shangdong Province Blood Center Jinan Blood Station, Jinan 250001, China;
3. Department of Biochemistry and Molecular Biology, School of Medicine, Shandong University, Jinan 250012, China

Received:2011-05-17 Online:2011-11-10 Published:2011-11-10

摘要/Abstract

摘要：

目的观察白藜芦醇对体外培养的人外周血单个核细胞(PBMCs)分泌白细胞介素-1β(IL-1β)、白细胞介素6(IL6)，单核细胞趋化蛋白1(MCP1)及对细胞因子信号抑制因子1和3（SOCS1/3）表达的影响。方法实验分5组，分别用0、3.13、6.25、12.5、25μg/mL的白藜芦醇作用于PBMCs，用实时荧光定量PCR、ELISA实验测定不同浓度白藜芦醇对PBMCs分泌IL1β、IL6及MCP1的mRNA和蛋白的表达，测定SOCS1/3mRNA的表达。结果与对照组相比，应用白藜芦醇后PBMCs分泌IL1β、IL6及MCP1的量减少, SOCS1/3的量增多。在蛋白水平，白藜芦醇浓度为25μg/mL时，IL1β及IL6表达明显减少(P<0.05)，尤其浓度为6.25μg/mL到25μg/mL时，MCP1的表达明显减少(P<0.05)。在mRNA水平，各组的IL1β、IL6及MCP1表达均明显降低(P<0.05)，而SOCS1/3表达均明显增高（P<0.05）。结论白藜芦醇在剂量依赖性抑制PBMCs促炎性细胞因子IL1β、IL6及MCP1产生的同时，还可以促进免疫负调控分子SOCS1/3的mRNA表达，这可能是白藜芦醇的抗炎作用及作用机制之一。

关键词: 外周血单个核细胞；白藜芦醇；细胞因子；细胞因子信号抑制因子

Abstract:

Objective To explore the effects of resveratrol on expressions of interleukin-1(IL-1β), interleukin-6(IL-6), monocyte chemotactic protein-1(MCP-1) and suppressor of cytokine signaling1/3(SOCS1/3) in cultured human peripheral blood mononuclear cells (PBMCs). Methods Cultured PBMCs were treated with different concentrations of resveratrol (0, 3.1, 6.25, 12.5, 25μg/mL ). Expression of IL-1β, IL-6 and MCP-1 at both mRNA and protein levels, SOCS1/3 at mRNA level were determined using real-time quantitative PCR and ELISA. Results Compared with the control group（0μg/mL）, expression of IL-1β, IL-6 and MCP-1 were significantly suppressed by resveratrol, while expression of SOCS1/3 were increased (P<0.05). The levels of IL-1β and IL-6 proteins expression significantly decreased at the concentration of 25μg/mL(P<0.05), and the level of MCP-1 protein expression decreased in groups from 6.25μg/mL to 25μg/mL(P<0.05). IL-β, IL-6 and MCP-1 mRNA expression were significantly suppressed while SOCS1/3 mRNA expression was highly induced by resveratrol at all groups(P<0.05). Conclusion Resveratrol can inhibit expression of the pro-inflammatory cytokines IL-1β, IL-6, MCP-1 and induce expression of SOCS1/3 in PBMCs at a dose dependent manner, which further indicates its anti-inflammatory function in pre-clinical studies.

Key words: Peripheral blood mononuclear cells; Resveratrol; Cytokine; Suppressor of cytokine signaling

中图分类号:

R331.1

彭洁1，韩雪莹2，胡晓燕3，邓景惕3，郭媛1. 白藜芦醇对外周血单个核细胞中IL-1β、IL-6、MCP-1及SOCS1/3 表达的影响[J]. 山东大学学报(医学版), 2011, 49(11): 64-.

PENG Jie1, HAN Xue-ying2, HU Xiao-yan3, DENG Jing-ti3, GUO Yuan1. The effect of resveratrol on expression of IL-1β, IL-6, MCP-1 andSOCS1/3 in human peripheral blood mononuclear cells[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(11): 64-.

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