新疆哈萨克族食管癌c-myc的表达及其调控机制

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (11): 135-.

新疆哈萨克族食管癌c-myc的表达及其调控机制

陈艳1，李秀梅2，王洪江3，庞作良3，李卉4，姜孝芳4，马莉莉1，谌宏鸣5，李惠武4

新疆医科大学 1. 基础医学院生物化学与分子生物学教研室, 乌鲁木齐 830011;
2. 基础医学院形态中心, 乌鲁木齐 830011; 3. 附属肿瘤医院胸外科, 乌鲁木齐 830000;
4. 基础医学院科研中心, 乌鲁木齐 830011; 5.基础医学院组织胚胎学教研室, 乌鲁木齐 830011

收稿日期:2011-02-18 出版日期:2011-11-10 发布日期:2011-11-10
通讯作者: 李惠武，教授，博士生导师，主要从事新疆哈萨克族食管癌发病机制的研究。E-mail:huiwuli1234@163.com
作者简介:陈艳（1978-），女，博士研究生，讲师，主要从事哈萨克族食管癌发病机制研究。
基金资助:
国家自然科学基金资助项目（N030950012）

Expression of c-myc in esophageal cancer in the Kazak population in Xinjiang, China：implications for regulatory mechanisms

HEN Yan1， LI Xiu-mei2， WANG Hong-jiang3， PANG Zuo-liang3，
LI Hui4， JIANG Xiao-fang4， MA Li-li1， CHEN Hong-ming5， LI Hui-wu4

1. Department of Biochemistry and Molecular Biology, Basic Medical College, Xinjiang Medical University,
Urumqi 830011, China; 2. Center of Morphology, Basic Medical College, Xinjiang Medical University, Urumqi 830011, China;
3. Department ofThoracic Surgery, Affiliated Tumor Hospital, Xinjiang Medical University, Urumqi 830000, China;
4. Research Center, Basic Medical College, Xinjiang Medical University, Urumqi 830011, China；
5. Department of Histology and Embryology, Basic Medical College, Xingjiang Medical University, Urumqi 830011, China

Received:2011-02-18 Online:2011-11-10 Published:2011-11-10

摘要/Abstract

摘要：

目的本研究通过检测新疆哈萨克族食管癌组织中MEK1/2、ERK1/2、JAK1/2、 STAT3和cmyc的表达，探讨调控c-myc表达的主要信号传导通路。方法对52例新疆哈萨克族食管癌组织及远端正常组织，采用RT-PCR检测c-myc的表达，并在c-myc差异表达的标本中检测MEK1/2、ERK1/2、JAK1/2及STAT3的表达水平。结果 c-myc在肿瘤组织中显著高表达（P＜0.05）。其中，ERK2和STAT3均与cmyc的表达呈正相关（P＜0.05），而JAK1/2和ERK1则与c-myc的表达无关；但ERK2与MEK1、MEK2和STAT3的表达呈正相关（P＜0.05）；此外，ERK1与MEK1和MEK2的表达也呈正相关（P＜0.05）。结论在新疆哈萨克族食管癌发生发展过程中，可能通过MEK/ERK2/STAT3信号传导通路调控cmyc，而JAK/STAT3信号途径可能不是激活c-myc的主要途径。

关键词: 哈萨克族；食管癌；c-myc；MEK1/2；ERK1/2；STAT3

Abstract:

Objective To study expressions of MEK1/2, ERK1/2, JAK1/2, STAT3 and c-myc genes in esophageal cancer in the Kazak population in Xinjiang, China and to explore the regulation signaling pathways for c-myc. Methods RT-PCR was used to detect c-myc expression in esophageal cancer tissues and distal normal tissues in 52 cases. Expressions of MEK1/2, ERK1/2, JAK1/2 and STAT3 were also examined for those specimens with differentially expressed c-myc gene. Results Expression of c-myc at the mRNA level in cancer tissues was higher than normal ones (P＜0.05). A positive correlation in expression was observed for c-myc with ERK2 and STAT3 (P＜0.05), but not with JAK1/2 and ERK1. A positive correlation was also observed between ERK2 and MEK1 as well as between MEK2 and STAT3 (P＜0.05). In addition, a positive correlation was detected for ERK1 with both MEK1 and MEK2 (P＜0.05). Conclusion The MEK/ERK2/STAT3 signaling pathway may play important roles leading to c-myc overexpression during esophageal cancer development in the Kazak population, while the JAK/STAT3 signaling pathway may not be a major pathway for c-myc activation.

Key words: Esophageal Cancer; c-myc; MEK1/2； ERK1/2； STAT3

中图分类号:

R574.1

陈艳1，李秀梅2，王洪江3，庞作良3，李卉4，姜孝芳4，马莉莉1，谌宏鸣5，李惠武4. 新疆哈萨克族食管癌c-myc的表达及其调控机制[J]. 山东大学学报(医学版), 2011, 49(11): 135-.

HEN Yan1， LI Xiu-mei2， WANG Hong-jiang3， PANG Zuo-liang3， LI Hui4， JIANG Xiao-fang4， MA Li-li1， CHEN Hong-ming5， LI Hui-wu4. Expression of c-myc in esophageal cancer in the Kazak population in Xinjiang, China：implications for regulatory mechanisms[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(11): 135-.

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