束缚-浸水应激对大鼠疑核胆碱能及儿茶酚胺能神经元活动的影响

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (10): 103-106.

束缚-浸水应激对大鼠疑核胆碱能及儿茶酚胺能神经元活动的影响

赵东芹，艾洪滨

山东师范大学生命科学学院生理学教研室，济南 250014

收稿日期:2011-05-24 出版日期:2011-10-10 发布日期:2011-10-10
通讯作者: 艾洪滨(1951- )，男，教授，博导，主要从事神经生理学的研究。
作者简介:赵东芹(1978- )，女，讲师，博士，主要从事环境动物学和动物生理学的研究。
基金资助:
国家自然科学基金资助项目(30770277、 30970354、 31071920)。

Influence of restraint water-immersion stress on activities of cholinergic and catecholaminergic neurons in the nucleus ambiguous in rats

ZHAO Dong-qin, AI Hong-bin

Department of Physiology, School of Life Science, Shandong Normal University, Jinan 250014, China

Received:2011-05-24 Online:2011-10-10 Published:2011-10-10

摘要/Abstract

摘要：

目的研究束缚-浸水应激(RWIS)大鼠疑核(NA)中胆碱能、儿茶酚胺能神经元的活动情况。方法随机将10只雄性Wistar大鼠分为对照组和应激组(RWIS组)，采用Fos、胆碱乙酰转移酶(ChAT)或酪氨酸羟化酶(TH)免疫组织化学双标技术，统计对照组和应激组Fos、ChAT或TH单标阳性神经元以及Fos+ChAT或Fos+TH双标阳性神经元数目。结果 NA中ChAT免疫阳性神经元胞体呈梭形或多角形，有较多的TH阳性终末。与对照组相比，RWIS组大鼠NA大量神经元表达Fos(P<0.05)；RWIS组Fos+ChAT双标阳性神经元数目也较对照组显著增加(P<0.05)，占ChAT免疫阳性神经元的比例在对照组和RWIS组中分别为7.5%、29.8%(P<0.01)。结论 NA中胆碱能神经元参与RWIS调控过程，而这些神经元可能接受其他核团儿茶酚胺能神经元的纤维投射。

关键词: 应激；疑核；胆碱0乙酰转移酶；酪氨酸羟化酶； Fos

Abstract:

Objective To investigate activities of cholinergic and catecholaminergic neurons in the nucleus ambiguous (NA) in response to water-immersion stress (RWIS) in rats. Methods Ten rats were randomly divided into the control group and the RWIS group. Fos-immunoreactive (Fos-IR), choline acetyltransferase immunoreactive (ChAT-IR), tyrosine hydroxylase immunoreactive (TH-IR) and double labeled (Fos+ChAT-IR and Fos+TH-IR) neurons were counted using immunohistochemistry. Results In the NA, ChAT-IR neurons were fusiform or polygonal, with a large amount of TH-IR ending. Compared with unstressed rats, the number of Fos-IR neurons was dramatically increased in the NA of RWIS rats (P<0.05), and the number of Fos+ChAT-IR neurons was greater in RWIS rats than unstressed rats (P<0.05). The percentage of Fos-IR nuclei in ChAT-IR neurons in unstressed and RWIS rats were 7.5% and 29.8%, respectively (P<0.01). Conclusion Cholinergic neurons in the NA, which might be regulated by catecholaminergic neurons in other nuclei, are involved in the RWIS response.

Key words: Stress; Nucleus ambiguous; Choline 0-acetyltransferase; Tyrosine hydroxylase; Fos

中图分类号:

赵东芹，艾洪滨. 束缚-浸水应激对大鼠疑核胆碱能及儿茶酚胺能神经元活动的影响[J]. 山东大学学报(医学版), 2011, 49(10): 103-106.

ZHAO Dong-qin, AI Hong-bin. Influence of restraint water-immersion stress on activities of cholinergic and catecholaminergic neurons in the nucleus ambiguous in rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(10): 103-106.

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