肾衰宁对糖尿病大鼠肾脏损伤的保护作用

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (1): 48-52.

肾衰宁对糖尿病大鼠肾脏损伤的保护作用

范例1，高洁1，刘茂静1，甄军晖2，姜虹3, 胡昭1

山东大学齐鲁医院 1.肾内科, 2.病理科,
3. 教育部和卫生部心血管重构与功能研究重点实验室，济南 250012

收稿日期:2010-08-05 出版日期:2011-01-10 发布日期:2011-01-10
通讯作者: 胡昭（1962- ），男，主任医师，硕士生导师，主要从事慢性肾脏疾病的研究。 E-mail: sdhuzhao2010@163.com
作者简介:范例（1983- ），女，硕士研究生，主要从事糖尿病肾病的研究。
基金资助:
国家科技支撑计划资助项目（２００７ＢＡＩ０４Ｂ１０）。

Protective effect of Shenshuaining on kidney injury in diabetic rats

FAN Li1, GAO Jie1, LIU Mao-jing1, ZHEN Jun-hui2, JIANG Hong3, HU Zhao1

QiLu Hospital of ShanDong University 1. Department of Nephrology, 2. Department of Pathology,
3. Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of
Education and Chinese Ministry of Public Health, Jinan 250012, China

Received:2010-08-05 Online:2011-01-10 Published:2011-01-10

摘要/Abstract

摘要：

目的探讨肾衰宁对糖尿病大鼠肾脏的保护作用及可能机制。方法雄性Vista大鼠24只，随机选取8只作为正常对照组（N组），其余16只注射链脲佐菌素（STZ）制成糖尿病模型，并随机分为糖尿病肾病组（DN组，n=8）和肾衰宁治疗组（S组，n=8），给药8周。检测血糖、肾功能、24h尿蛋白定量等指标，并进行肾脏病理学检查及免疫组化测定肾组织中转化生长因子β1（TGF-β1 ）、层黏连蛋白（LN）及Ⅳ型胶原蛋白（Col Ⅳ）的表达。结果与N组相比，DN组和S组血糖、血肌酐、尿素氮、24h尿蛋白均明显增加（P<0.01），肾组织中TGF-β1 、LN及Col Ⅳ表达明显升高（P<0.01）；与DN组相比，S组除血糖外（P>0.05），其余指标均明显降低（P<0.01）。结论肾衰宁在糖尿病的肾损伤过程中具有保护作用，可降低尿蛋白，改善肾功能，并通过下调肾脏TGF-β1的表达，抑制细胞外基质（ECM）成分的积聚，从而延缓肾脏纤维化的进程。

关键词: 糖尿病，肾性；肾衰宁；转化生长因子β1；层黏连蛋白；Ⅳ型胶原蛋白

Abstract:

Objective To investigate the protective effect and mechanism of Shenshuaining on kidney injury in diabetic rats. Methods Of 24 male vista rats, 8 rats were randomly selected as the control group(N group), while the other 16 rats were induced into the diabetic models by a single intraperitoneal streptozotocia(STZ) injection. Then, the latter group was randomly divided into the diabetic nephropathy group(DN group,n=8) and the Shenshuaining treating group(S group,n=8). The therapy lasted for 8 weeks. Blood glucose, renal function and 24h urinary protein excretion quantity were detected. Renal pathological examination was observed and expressions of transforming growth factorbeta l(TGF-β1 ), laminin(LN ) and collagen IV(Col Ⅳ) in nephridial tissues were measured by immunohistochemistry. Results Compared with the N group, the levels of blood glucose, SCr, BUN and 24h urine protein excretion were significantly increased in the DN and S groups (P<0.01), and so were expressions of TGF-β1, LN and Col Ⅳ in nephridial tissues (P<0.01). Compared with the DN group, all the above parameters were significantly decreased in the S group (P<0.01), except for the level of blood glucose(P>0.05). Conclusion Shenshuaining has a protective effect on kidney injury in diabetic rats. It can reduce urine protein excretion, improve renal function, down-regulate expression of TGF-β1 and inhibit the accumulation the extracellular matrix(ECM) to slow down the process of renal fibrosis.

Key words: Diabetes, renal; Shenshuaining; Transforming growth factor-beta l; Laminin; Collagen IV

中图分类号:

R692

范例1，高洁1，刘茂静1，甄军晖2，姜虹3, 胡昭1. 肾衰宁对糖尿病大鼠肾脏损伤的保护作用[J]. 山东大学学报(医学版), 2011, 49(1): 48-52.

FAN Li1, GAO Jie1, LIU Mao-jing1, ZHEN Jun-hui2, JIANG Hong3, HU Zhao1. Protective effect of Shenshuaining on kidney injury in diabetic rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(1): 48-52.

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