泛素结合酶-10表达对结直肠癌细胞增殖的影响

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (1): 35-38.

泛素结合酶-10表达对结直肠癌细胞增殖的影响

陈世敏1，2，辛家璇1，徐春晓1，刘斌1，孙晓明2，胡成进2，刘贤锡1

1.山东大学医学院生物化学与分子生物学研究所，济南 250012；
2.济南军区总医院实验诊断科，济南 250031

收稿日期:2009-12-26 出版日期:2011-01-10 发布日期:2011-01-10
通讯作者: 刘贤锡（1946- ），教授，博士生导师，主要从事肿瘤分子生物学研究。 E-mail: xianxi@sdu.edu.cn
作者简介:陈世敏（1971- ），博士，主管技师，主要从事肿瘤分子生物学研究。
基金资助:
山东省科技发展计划资助项目（2008GG30002030）。

Effects of UbcH10 expression on proliferation of colorectal cancer cells

CHEN Shi-min1，2, XIN Jia-xuan1, XU Chun-xiao1, LIU Bin1, SUN Xiao-ming2, HU Cheng-jin2, LIU Xian-xi1

1. Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University, Jinan 250012, China;
2. Department of Laboratory Diagnosis, General Hospital of Jinan Military Region, Jinan 250031, China

Received:2009-12-26 Online:2011-01-10 Published:2011-01-10

摘要/Abstract

摘要：

目的探讨泛素结合酶-10（Ubiquitinconjugating enzymes， UbcH10）表达对结直肠癌细胞增殖的影响。方法采用脂质体LipofectamineTM 2000转染人UbcH10的真核表达载体pcDNA3.1UbcH10及其对照载体pcDNA3.1至结直肠癌细胞LoVo，蛋白印迹（Western blot）技术分析UbcH10的表达；细胞增殖试验（CCK-8试剂）检测UbcH10表达对LoVo细胞增殖的影响；流式细胞术检测UbcH10表达对LoVo细胞周期的影响。结果 Western blot分析证实，与对照组细胞相比，转染重组质粒的LoVo细胞中UbcH10表达明显升高；细胞生长增殖曲线显示，UbcH10过度表达的细胞生长明显快于对照组细胞。流式细胞术分析细胞周期结果显示：与对照组相比，UbcH10过度表达组细胞在G2-M期分布明显减少（P＜0.05）。结论 UbcH10能促进结直肠癌细胞增殖，调控细胞周期进展可能是其潜在的作用机制。

关键词: 泛素结合酶；细胞增殖；细胞周期

Abstract:

Objective To investigate the effect of the UbcH10(Ubiquitin-conjugating enzymes) expression on the proliferation of colorectal cancer cells. Methods The pcDNA3.1UbcH10 and pcDNA3.1 were transfected into LoVo cells. UbcH10 expression was determined by Western blot. Proliferation of LoVo cells was detected by CCK-8 kit. The effect of UbcH10 on the cell cycle of colorectal cancer cells was assessed by flow cytometry. Results The UbcH10 expression in LoVo cells transfected with pcDNA3.1UbcH10 was more obviously increased than that in control cells. Over-expression of UbcH10 obviously promoted cell proliferation compared with the controls. UbcH10 over-expression significantly decreased cell cycle distribution in the G2-M phase (P<0.05). Conclusion UbcH10 can promote proliferation of colorectal cancer cells. The potential mechanism may depend on its regulation of the cell cycle.

Key words: Ubiquitin-conjugating enzymes; Cell proliferation; Cell cycle

中图分类号:

R318

陈世敏1，2，辛家璇1，徐春晓1，刘斌1，孙晓明2，胡成进2，刘贤锡1. 泛素结合酶-10表达对结直肠癌细胞增殖的影响[J]. 山东大学学报(医学版), 2011, 49(1): 35-38.

CHEN Shi-min1，2, XIN Jia-xuan1, XU Chun-xiao1, LIU Bin1, SUN Xiao-ming2, HU Cheng-jin2, LIU Xian-xi1. Effects of UbcH10 expression on proliferation of colorectal cancer cells[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(1): 35-38.

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