慢性HCV感染患者外周血CD81、CD4表达的观测

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (9): 89-.

慢性HCV感染患者外周血CD81、CD4表达的观测

程广霞1，2，张玲1，刘靓雯2，冯照雷2，李翠玲1

1.山东省医学科学院基础医学研究所，山东省医药卫生肿瘤免疫与中药免疫重点实验室，
山东省现代医用药物与技术重点实验室，济南 250062；
2.济南市传染病医院检验中心，济南 250021)摘要：目的研究慢性丙型肝炎病毒（HCV）感染患者外周血CD81、CD4的表达，探讨CD81对患者机体免疫系统的调节作用。方法选择HCV感染患者55例(感染组

收稿日期:2010-03-29 出版日期:2010-09-16 发布日期:2010-09-16
通讯作者: 张玲（1950- ），女，研究员，主要从事免疫调控和抗肿瘤研究。 E-mail：zhlnln@tom.com
作者简介:程广霞（1980- ），女，医学硕士，主管检验师，主要从事免疫学研究。

Peripheral expressions CD81 and CD4 in chronic HCV patients

CHENG Guang-xia1,2, ZHANG Ling1, LIU Jing-wen2, FENG Zhao-lei2, LI Cui-ling1

1. Institute of Basic Medicine Shandong Academy of Medical Sciences; Medical Health Key Laboratory for Tumor Immunology
and Chinese Medicine Immunology of Shandong Province; Key Laboratory for Modern Medicine and Technology of
Shandong Province, Jinan 250062， China; 2. Laboratory Center of Jinan Infectious Disease Hospital, Jinan 250021， China

Received:2010-03-29 Online:2010-09-16 Published:2010-09-16

摘要/Abstract

摘要：

目的研究慢性丙型肝炎病毒（HCV）感染患者外周血CD81、CD4的表达，探讨CD81对患者机体免疫系统的调节作用。方法选择HCV感染患者55例(感染组)，健康对照25例（健康对照组），采用流式细胞术检测其外周血CD81、CD4的表达水平，ELISA法检测血清中白介素 2（IL2）的含量， RT-PCR法检测血清中HCV RNA的含量。结果感染组CD81分子的表达水平与血清中HCV RNA的含量呈正相关（r=0.474， P＜0.01）。感染组外周血淋巴细胞中CD81+细胞和CD3+CD4+T细胞中CD3+CD4+CD81+T细胞的数量均高于健康对照组（P＜0.01），且二者呈正相关（r=0.581， P＜0.01）；感染组CD3+CD4+T细胞的数量和血清中IL2的含量均显著低于健康对照组（P＜0.05），且两者呈正相关（r=0.378，P＜0.05）。结论 HCV感染后，HCV RNA的持续存在可能促进细胞表面CD81分子的表达；CD81分子高表达的同时，伴随CD3+CD4+T细胞数量降低，且分泌的细胞因子IL-2减少，参与患者机体的免疫调节。

关键词: 丙型肝炎病毒；白细胞分化抗原81；CD3+CD4+T细胞；白细胞介素2；丙型肝炎病毒RNA

Abstract:

Objective To investigate peripheral CD81 and CD4 expressions in patients infected by chronic Hepatitis C Virus (HCV), and explore the modulation effect of CD81 on the immune system. Methods Samples were collected from 55 chronic hepatitis C patients (infection group) and 25 healthy persons (control group). Peripheral expression of CD81 and CD4 were measured by flow cytometry. Serum level of IL2 and HCV RNA were detected by ELISA and RTPCR, respectively. Results The peripheral level of CD81+ cells and the serum level of HCV RNA were positively correlated(r=0.474, P＜0.01) in the infection group. The numbers of the peripheral CD81+ lymphocytes and the CD3+CD4+ CD81+T cells among CD3+CD4+ T cells in the infection group were higher than those in the control group (P＜0.01). Both were positively correlated(r=0.581,P＜0.01). Also, the number of peripheral CD3+CD4+T cells and the serum level of IL2 in the infection group were considerably lower than those in the control group (P＜0.05). Both were also positively correlated(r=0.378, P＜0.05). Conclusion After HCV infection, the consistent presence of HCV RNA can promote expression of CD81 while number of CD3+CD4+T cells and secretion of IL-2 decline, which participate in the immuno-regulation of the patients.

Key words: Hepatitis C virus; Cluster of differentiation 81; CD3+CD4+T cells; Interleukin-2; Hepatitis C virus RNA

中图分类号:

R512.63

程广霞1，2，张玲1，刘靓雯2，冯照雷2，李翠玲1. 慢性HCV感染患者外周血CD81、CD4表达的观测[J]. 山东大学学报(医学版), 2010, 48(9): 89-.

CHENG Guang-xia1,2, ZHANG Ling1, LIU Jing-wen2, FENG Zhao-lei2, LI Cui-ling1. Peripheral expressions CD81 and CD4 in chronic HCV patients[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(9): 89-.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed