血小板衍生生长因子对人脐带间充质干细胞胞外基质的影响

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (9): 59-63.

血小板衍生生长因子对人脐带间充质干细胞胞外基质的影响

庄泳1, 李栋2, 时庆2, 汪大琨2, 蔡直锋1, 鞠秀丽1

山东大学齐鲁医院 1.儿科；2．低温医学研究室，济南 250012

收稿日期:2010-03-29 出版日期:2010-09-16 发布日期:2010-09-16
通讯作者: 鞠秀丽（1964- ），女，教授，博士生导师，主要从事儿科血液学专业研究。 E-mail: shellysdcn07@hotmail.com
作者简介:庄泳（1984- ），男，硕士研究生，主要从事儿科血液学专业研究。
基金资助:
国家自然科学基金资助项目(30700163)；山东省自然科学基金资助项目（Y2007C109）；山东省科技攻关计划资助项目（2009GG10002027）。

Effects of the plateletderived growth factor on proliferation and extracellularmatrix of human umbilical cord mesenchymal stem cells

ZHUANG Yong1, LI Dong2, SHI Qing2, WANG Da-kun2, CAI Zhi-feng1, JU Xiu-li1

1. Department of Pediatrics; 2. Cryomedicine Lab, Qilu Hospital of Shandong University, Jinan 250012, China

Received:2010-03-29 Online:2010-09-16 Published:2010-09-16

摘要/Abstract

摘要：

目的探讨血小板衍生生长因子(PDGF)对人脐带间充质干细胞(UCMSCs)增殖和细胞外基质(ECM)表达的影响及其可能的作用机制。方法体外传代培养UCMSCs，分为对照组和实验组，其中对照组不加PDGF，实验组分别加入不同质量浓度(2、5、10、20、50μg/L)的PDGF。加入PDGF 12h后，用qRT-PCR法检测ECM基因和凋亡相关基因(Bcl2、Bax)的表达；加入PDGF 24h后，用四甲基偶氮唑(MTT)法检测细胞增殖。结果与对照组比较，随着PDGF质量浓度的增加，反映细胞代谢活性的OD值逐渐增加，且PDGF质量浓度在20μg/L时达到最高值，但PDGF质量浓度为50μg/L时OD值反而降低；PDGF质量浓度为2μg/L时，ECM表达和Bcl2/Bax比值较对照组有所降低，随着PDGF质量浓度的增加，ECM的表达和Bcl2/Bax比值呈现先增高后降低的趋势，且在10μg/L时达到最高值。结论 PDGF可以促进脐带间充质干细胞的增殖，同时能促进其ECM的表达，还能提高细胞耐受凋亡的能力，最佳作用质量浓度为10μg/L。

关键词: 脐带；间充质干细胞；血小板衍生生长因子；细胞外基质

Abstract:

Objective To investigate the effects of the platelet-derived growth factor(PDGF) on the proliferation, extracellular matrix synthesis and its probable mechanism in human umbilical cord mesenchymal stem cells(UCMSCs). Methods Cultured UCMSCs were divided into 6 groups: the control group, and the PDGF-treated groups(2, 5, 10, 20 and 50μg/L). Morphological changes of the cells were observed under an inverted microscope. MTT assay was used to detect the PDGF effects on cell proliferation. Expressions of ECM mRNA and apoptosis-related genes(Bcl-2, Bax)were detected by qRT-PCR. Results With an increased concentration of PDGF, UCMSCs proliferation was gradually promoted compared with the control group. 20μg/L PDGF greatly promoted UCMSCs proliferation, however, 50μg/L showed inhibitive effects. Expressions of ECM mRNA and apoptosis-related factor (Bcl-2/Bax) were down-regulated in the 2μg/L PDGF group but up-regulated when PDGF≥5μg/L. 10μg/L PDGF greatly upregulated ECM mRNA expression and apoptosis-related factor (Bcl2/Bax). Conclusion PDGF can promote the proliferation of UCMSCs, stimulate ECM synthesis and improve the ability to withstand apoptosis of UCMSCs in vitro. 10μg/L PDGF was the most effective concentration.

Key words: Cord; Mesenchymal stem cells; Plateletderived growth factor; Extracellular matrix

中图分类号:

Q291

庄泳1, 李栋2, 时庆2, 汪大琨2, 蔡直锋1, 鞠秀丽1. 血小板衍生生长因子对人脐带间充质干细胞胞外基质的影响[J]. 山东大学学报(医学版), 2010, 48(9): 59-63.

ZHUANG Yong1, LI Dong2, SHI Qing2, WANG Da-kun2, CAI Zhi-feng1, JU Xiu-li1. Effects of the plateletderived growth factor on proliferation and extracellularmatrix of human umbilical cord mesenchymal stem cells[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(9): 59-63.

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