关键词: 紫草；muc1蛋白；子宫内膜；抗胚胎植入

Abstract:

Objective     To explore the relationship between anti-implantation effect of arnebia root and expression of the muc1 protein in the endometrium of early pregnant mice.  Methods    Mated female mice were randomly divided into the experimental groups and the control group. The experimental groups were fed with an arnebia root solution at different concentrations and the control group was fed with saline on day 1，2, 3, 4 of pregnancy. The uteri of the mice were removed at night on day 4 of pregnancy. In order to observe the morphological changes and expression of the muc1 protein in the endometrium, one side of the uteri were stained with HE and immunohistochemistry. Expression of the muc1 protein on the other side of the uteri was measured by Western blot. The mated mice were executed to assess the implantation rate on day 8 of pregnancy. Results     The endometrium in the experimental groups exhibited morphological changes compared with that in the control group. Expression of the muc1 protein was positively related to the doses of arnebia root, and the difference between experimental groups and the control group was significant（P＜0.05）.The rates of embryo implantation in the experimental groups and the control group were significantly different（P＜0.05）. Conclusion    The arnebia root can prevent embryo implantation by inhibiting disappearance of the muc1 protein.

Key words: Arnebia root; Mucin 1 protein; Endometrium; Anti-implantation