关键词: 鼻咽肿瘤；内皮/上皮细胞酪氨酸激酶；细胞增殖

Abstract:

Objective    To study the relationship between the tyrosine kinases Etk/BMX and nasopharyngeal carcinoma (NPC) cell proliferation. Methods    Wild type Etk/BMX pcDNA3.1Etk was recombinated into low expression of Etk/BMX NPC cell line sune-1 using plasmid 2000, and thus the sune-wt cell line which Etk was highly and stably expressed was established, and cells which transfected by pcDNA3.1 empty plasmid (sune-vector) was used as controls.The transfection rate was examined using flow cytometry and then the cells were detected  by a clone plate experiment and inoculated  into nude mice  to observe tumorigenesis. Results    The sune-wt cells with high Etk expression were successfully obtained, showing an expression rate of (75.5±2.34)%. Clone plate experiment showed that grow rate of sune-wt cells was higher than that of sune-vector and sune-1 cells. sune-wt had a higher rate of growth and tumorigenesis than both sune-vector and sune-1 cells in nude mice，but there were no significant differences in tumor growth between sune-vector and sune-1 cells. Conclusion    Etk/BMX may increase the oncogenicity and cell proliferation in nasopharyngeal carcinoma.

Key words:  Nasopharyngeal carcinoma;   Endothelial/epithelial tyrosine kinase; Cell proliferation