肝性脑病大鼠肝及脑组织蛋白质硝基化和细胞凋亡的研究

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (8): 38-41.

肝性脑病大鼠肝及脑组织蛋白质硝基化和细胞凋亡的研究

叶青1，荣琳2，汪心婷3，邵丽华2

山东大学 1.医学院； 2.公共卫生学院卫生检验研究所； 3.公共卫生学院流行病与卫生统计学研究所，济南 250012

收稿日期:2010-05-23 出版日期:2010-08-16 发布日期:2010-08-16
通讯作者: 邵丽华，教授，主要从事氧化/硝基化应激与疾病关系研究。 E-mail：slh@sdu.edu.cn
作者简介:叶青（1986- ），女，本科生，主要从事氧化/硝基化应激与疾病关系研究。
基金资助:
山东省自然科学基金资助课题（Y2007C079）。

Protein nitrotyrosine and cell apoptosis in the liver and brain of hepatic encephalopathy rats

YE Qing1, RONG Lin2, WANG Xin-ting3, SHAO Li-hua2

1. School of Medicine; 2. Institute of Chemistry and Microbacteria Detection, School of Public Health; 3. Institute of Epidemiology and Health Statistics, School of Public Health， Shandong University， Jinan 250012， China

Received:2010-05-23 Online:2010-08-16 Published:2010-08-16

摘要/Abstract

摘要：

目的探讨肝性脑病（HE）大鼠肝脏及脑组织中的蛋白质硝基化和细胞凋亡与HE发病的关系。方法雄性Wistar大鼠随机分为HE组和空白对照组。HE组用硫代乙酰胺（TAA）制造大鼠HE模型。采用链霉亲和素-生物素-酶复合物（SABC）免疫组化法检测肝脏和脑组织硝基化蛋白质和一氧化氮合酶（NOS）；用原位末端脱氧核糖核酸转移酶标记法（TUNEL）检测细胞凋亡，并计算凋亡指数；空白对照组大鼠用生理盐水做平行对照。结果 HE组大鼠肝脏和脑组织中检测到明显的硝基化蛋白质沉积，而对照组少见；HE组大鼠肝脏和脑组织中检测到NOS的表达，对照组甚少；HE组大鼠肝脏和脑组织细胞凋亡指数较对照组明显增高（P<0.01）。结论 HE与其肝脏和脑组织蛋白质硝基化关系密切。

关键词: 肝性脑病；蛋白质酪氨酸硝基化；细胞凋亡；大鼠，Wistar

Abstract:

Objective To investigate protein nitrotyrosine and cell apoptosis in the liver and brain tissues of rat model of hepatic encephalopathy (HE). Methods Male Wistar rats were randomly divided into HE group and control group. To manufacture the HE model, the HE group was treated with thioacetamide (TAA) by intraperitoneal injection. The control group was treated with normal saline by the same way. Then frozen sections were made from the rat liver and brain of the 2 groups. In the frozen sections, protein nitrotyrosine (3-NT) and nitricoxide synthase (NOS) was tested by immunoenzymic method of SABC, and cell apoptosis was tested by TdT-mediated dUTP nick-end labeling（TUNEL）. Cell apoptosis index was calculated. Results Significant nitrate protein deposition was observed in the rat liver frozen sections in the HE group, while it was rare in the control group. The expression of NOS was much more in the rat liver and brain tissues of the HE group. Cell apoptosis index was significantly higher in the HE group than in the control group （P<0.01）. Conclusions There is a close relationship between HE and protein nitration in the liver and brain tissues.

Key words: Hepatic encephalopathy; Protein nitrotyrosine; Apoptosis; Rats, Wistar

中图分类号:

R575.3

叶青1，荣琳2，汪心婷3，邵丽华2. 肝性脑病大鼠肝及脑组织蛋白质硝基化和细胞凋亡的研究[J]. 山东大学学报(医学版), 2010, 48(8): 38-41.

YE Qing1, RONG Lin2, WANG Xin-ting3, SHAO Li-hua2. Protein nitrotyrosine and cell apoptosis in the liver and brain of hepatic encephalopathy rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(8): 38-41.

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