山东产半夏药材的高效液相指纹图谱分析

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (8): 120-123.

山东产半夏药材的高效液相指纹图谱分析

李斌，程秀民，周永妍，娄红祥

山东大学药学院分析测试中心，济南 250012

收稿日期:2010-03-17 出版日期:2010-08-16 发布日期:2010-08-16
通讯作者: 程秀民（1951- ），男，教授。主要从事中草药有效成分提取和中药色谱分析。 E-mail:minn@sdu.edu.cn
作者简介:李斌（1986- ），女，硕士研究生，主要从事仪器分析和中药色谱分析。
基金资助:
国家支撑计划资助项目(2006BAI06A01-05)。

Fingerprint of Shandong trueborn Pinellia ternata(Thunb.) Breit. analyzed by HPLC

LI Bin, CHENG Xiu-min, ZHOU Yong-yan, LOU Hong-xiang

Pharmaceutical Analysis Center, School of Pharmaceutical sciences, Shandong University, Jinan 250012, China

Received:2010-03-17 Online:2010-08-16 Published:2010-08-16

摘要/Abstract

摘要：

目的建立山东产半夏药材的HPLC指纹图谱，为半夏药材质量控制提供方法和依据。方法采用C18柱（250mm×4.6mm,5μm），以乙腈—水作为流动相进行梯度洗脱，流速1.0mL/min，检测波长为260nm，对获得的HPLC指纹图谱采用相似度评价软件和SPSS13.0软件进行相似度评价和聚类分析。结果建立了山东产半夏药材的HPLC指纹图谱，方法学考察符合指纹图谱技术要求。相似度计算及聚类分析表明，山东不同产区半夏药材指纹图谱之间相似度高，归为一大类，但与商品半夏药材相似度差异较大、与半夏炮制品、天南星等则不能聚为一类。结论建立的半夏药材高效液相分析方法简单、准确、重现性好，峰分离度较好，可作为半夏药材鉴别和量化控制的依据。

关键词: 半夏；色谱法；高压液相；相似度评价； SPSS统计分析

Abstract:

Objective To establish a high-performance liquid chromatography (HPLC) fingerprint of Shandong trueborn Pinellia ternata (Thunb.) Breit. for quality control. Methods HPLC was used by ODS column(4.6mm×250mm, 5μm) with the gradient elution solvert system composed of acetonitrile-water. The flow rate was 1.0mL/min, and the detection wavelength was 260nm. The HPLC fingerprints were compared by the similarity evaluation software and SPSS software. Results The HPLC fingerprint of Shandong trueborn Pinellia was established, and methods established for Pinellias conformed to the technical standards in fingerprint spectrum. All the samples from Shandong Province were similar and were clustered into one group, and they were different from the commodity pinellia. Conclusion The established HPLC fingerprint has desirable accuracy, repeatability and stability. It can be used for the quality control of Pinellia ternata (Thunb.) Breit.

Key words: Pinellia ternata (Thunb.) Breit.; Stratography, High-performance liquid chromatography; Similarity evaluation; SPSS statistical analysis

中图分类号:

R284.1

李斌，程秀民，周永妍，娄红祥. 山东产半夏药材的高效液相指纹图谱分析[J]. 山东大学学报(医学版), 2010, 48(8): 120-123.

LI Bin, CHENG Xiu-min, ZHOU Yong-yan, LOU Hong-xiang . Fingerprint of Shandong trueborn Pinellia ternata(Thunb.) Breit. analyzed by HPLC[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(8): 120-123.

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