地塞米松对平阳霉素致肺纤维化大鼠肺组织病理形态、超微结构及血清TGF-β1、IL-8动态变化的影响

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (7): 27-32.

地塞米松对平阳霉素致肺纤维化大鼠肺组织病理形态、超微结构及血清TGF-β1、IL-8动态变化的影响

王凤强1,王爱华1,尹迎秋2,王荣3

1.山东大学齐鲁医院呼吸内科，济南 250012；
2.粤北人民医院呼吸内科，广东韶关512000；
3.教育部、卫生部心血管重构与功能研究重点实验室，济南 250012

收稿日期:2010-04-15 出版日期:2010-07-16 发布日期:2010-07-16
通讯作者: 王爱华（1966- ），女，副教授，硕士生导师，主要从事肺间质疾病的研究。 Email：wah966@yahoo.com.cn
作者简介:王凤强（1981- ），男，硕士研究生，研究方向肺间质纤维化。 Email：wangfengqiang8341@qq.com
基金资助:
山东省自然基金项目(26010105200605)

Effects of dexamethasone on dynamic changes of lung tissue pathology, ultrastructure and levels of TGFβ1 and IL8 in serum of Pingyangmycininduced pulmonary fibrosis in rats

WANG Feng-qiang1 ， WANG Ai-hua1， YIN Ying-qiu2， WANG Rong3

1. Department of Pulmonary Medicine, Qilu Hospital of Shandong University, Jinan 250012, China;
2. Department of Pulmonary Medicine, Yuebei People′s Hospital，Shaoguan 512000, Guangdong, China;
3. Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education, Chinese Ministry of Health, Jinan 250012, China

Received:2010-04-15 Online:2010-07-16 Published:2010-07-16

摘要/Abstract

摘要：

目的观察地塞米松对平阳霉素致肺纤维化大鼠肺组织病理形态、超微结构及血清转化生长因子β1（transforming growth factor-β1, TGF-β1）、白细胞介素8(interleukin-8,IL-8)动态变化的影响。方法将105只健康雄性Wistar大鼠随机分为对照组、模型组和地塞米松组，其中对照组25只，模型组和地塞米松组各40只。模型组和地塞米松组Wistar大鼠经气管穿刺注射平阳霉素5mg/kg复制大鼠肺纤维化模型，对照组Wistar大鼠经气管穿刺注射等体积生理盐水。造模后地塞米松组大鼠给予腹腔注射地塞米松(4mg/kg体重)，模型组和对照组给予等体积生理盐水，以上3组均1次/d。分别于1、7、14、21、28d取材，对照组每批5只，模型组和地塞米松组每批8只，检测肺组织病理改变,酶联免疫吸附试验 ( ELISA )检测血清TGF-β1 、IL-8水平。结果肺组织病理学检查结果符合肺纤维化的典型表现证实造模成功;模型组和地塞米松组超微结构较对照组明显改变;模型组和地塞米松组血清TGF-β1、IL-8水平先升高后降低, 各时间点均高于对照组，以第7天为著,差异有统计学意义,而模型组和地塞米松组间血清TGFβ1、IL-8差异均无统计学意义 (P>0.05)。结论 TGF-β1、IL-8在平阳霉素诱导的肺纤维化中起重要作用,参与了肺泡炎和肺纤维化过程。地塞米松(4mg/kg)并不能降低肺纤维化大鼠血清TGF-β1、IL-8水平,可能与地塞米松同时可抑制机体免疫,从而增加了罹患感染的机会有关。

关键词: 肺纤维化；平阳霉素；转化生长因子β1；白细胞介素8

Abstract:

Objective To investigate the effects of dexamethasone on dynamic changes of lung tissue pathology, ultrastructure and the levels of TGF-β1 and IL-8 in serum of Pingyangmycin-induced pulmonary fibrosis in rats. Methods 105 healthy male Wistar rats were randomly divided into three groups: the control group(n=25), the model group(n=40) and the dexamethasone group(n=40). Rats in both the model group and the dexamethasone group were injected with Pingyangmycin(5mg/kg)through tracheofistulization to induce pulmonary fibrosis, while rats in the control group were injected with equivalent physiological saline. After operation, dexamethasone (4mg/kg body weight) was intraperitoneally subjected to the dexamethasone group rats once per day. Equivalent saline was administered to rats in both the control group and the model group. On the 1st, 7th, 14th, 21st and 28th day, five rats in the control group and eight rats in both of the other two groups were dissected to observe the pathological changes of the lung tissues. The ultrastructure and the levels of TGF-β1 and IL-8 in serum were determined by enzymelinked immunosorbent assay. Results Histopathologic examination results were consistent with the characteristics of rat pulmonary fibrosis models. The lung ultrastructures of the model group and the dexamethasone group significantly changed compared with the control group. At different time points, the concentrations of TGF-β1 and IL-8 in serum were increased and then decreased in both the model group and the dexamethasone group, and were significantly higher than those of the control group, especially on the 7th day. Levels of TGF-β1 and IL-8 in serum were similar between the model group and the dexamethasone group, and there were no significant difference between them (P>0.05) . Conclusion TGF-β1 and IL-8 play an important role in the process of alveolitis and the pulmonary fibrosis induced by Pingyangmycin in rats. Dexamethasone (4mg/kg) does not reduce the levels of TGF-β1 and IL-8 in serum of Pingyangmycin-induced pulmonary fibrosis in rats, this may be associated with the immunocompromised state, which increases the risk of the chance of infection.

Key words: Pulmonary fibrosis; Pingyangmycin; TGF-β1; Interleukin-8

中图分类号:

R563

王凤强1,王爱华1,尹迎秋2,王荣3. 地塞米松对平阳霉素致肺纤维化大鼠肺组织病理形态、超微结构及血清TGF-β1、IL-8动态变化的影响[J]. 山东大学学报(医学版), 2010, 48(7): 27-32.

WANG Feng-qiang1 ， WANG Ai-hua1， YIN Ying-qiu2， WANG Rong3. Effects of dexamethasone on dynamic changes of lung tissue pathology, ultrastructure and levels of TGFβ1 and IL8 in serum of Pingyangmycininduced pulmonary fibrosis in rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(7): 27-32.

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