慢性心力衰竭患者外周血中Th17及Treg细胞的检测及意义

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (6): 100-103.

慢性心力衰竭患者外周血中Th17及Treg细胞的检测及意义

李娜，边红军，张娟，季晓平

教育部和卫生部心血管重构与功能研究重点实验室，山东大学齐鲁医院心内科，济南 250012

收稿日期:2010-01-02 出版日期:2010-06-16 发布日期:2010-06-16
通讯作者: 季晓平(1964- )，男，博士生导师，主要从事心力衰竭的分子生物学研究。 Email:xpj64@163.com
作者简介:李娜(1985- )，女，硕士研究生，研究方向为心力衰竭的免疫学机制

Detection and significance of Th17 and Treg cells in patients with chronic heart failure

LI Na, BIAN Hongjun, ZHANG Juan, JI Xiaoping

Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of
Public Health, Department of Cardiology, Qilu Hospital of Shandong University, Jinan 250012, China

Received:2010-01-02 Online:2010-06-16 Published:2010-06-16

摘要/Abstract

摘要：

目的探讨慢性心力衰竭(CHF)患者外周血中Th17细胞、CD4+CD25+Foxp3+调节性T细胞(Treg)的变化及意义。方法 49例CHF患者按照NYHA心功能分级分为CHF1组(NYHA心功能Ⅰ～Ⅱ级，n=21)和CHF2组(NYHA心功能Ⅲ～Ⅳ级，n=28)。18例健康体检者作为对照组。采用流式细胞分析法检测外周血中Th17、Treg细胞占CD4+T细胞的比例。结果 CHF1组和CHF2组患者外周血中Th17/CD4+T细胞的比例明显高于对照组(P均<0.01)，CHF2组亦明显高于CHF1组(P<0.01)；CHF1组和CHF2组患者外周血中Treg/CD4+T细胞的比例明显低于对照组(P均<0.01)，CHF2组亦明显低于CHF1组(P<0.05)；Th17/Treg的比值随心功能的减低而增高(P<0.01)。结论 CHF患者外周血中存在Th17/Treg失衡，且与心功能有一定关系，Th17/Treg失衡可能参与了CHF的发生发展。

关键词: 心力衰竭;Th17细胞;CD4+CD25+Foxp3+T细胞

Abstract:

Objective To investigate the change of Th17 cells and CD4+CD25+Foxp3+ regulatory T cells (Treg) in patients with chronic heart failure (CHF). Methods The frequencies of Th17 cells and Treg cells were detected in 49 patients with CHF and 18 healthy controls by flow cytometry. And the 49 patients were divided into 2 groups according to NYHA heart function class: CHF1 group (NYHAⅠ-Ⅱ, n=21) and CHF2 group (NYHAⅢ-Ⅳ, n=28). Results The frequencies of Th17 cells were significantly higher in patients with poor heart function (CHF1 group and CHF2 group) than in those of normal heart function (the healthy controls) (P<0.01), and the frequencies in CHF2 group were significantly higher than in CHF1 group (P<0.01). But the change of Treg cells was totally opposite. The frequencies of Treg cells were significantly lower in CHF1 group and CHF2 group than in the healthy controls (P<0.01), the frequencies in CHF2 group were significantly lower than in CHF1 group (P<0.05), and we confirmed that the ratios of Th17/Treg increased with decrease of the heart function (P<0.01). Conclusion Th17/Treg imbalance existed in patients with CHF, so it may play a potential role in the onset and development of CHF.

Key words: Heart failure; Th17 cells; CD4+CD25+Foxp3+T cells

中图分类号:

R541.6

李娜，边红军，张娟，季晓平. 慢性心力衰竭患者外周血中Th17及Treg细胞的检测及意义[J]. 山东大学学报(医学版), 2010, 48(6): 100-103.

LI Na, BIAN Hongjun, ZHANG Juan, JI Xiaoping. Detection and significance of Th17 and Treg cells in patients with chronic heart failure[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(6): 100-103.

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