促黑素对小鼠骨骼肌细胞脂肪酸氧化的影响

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (5): 9-13.

促黑素对小鼠骨骼肌细胞脂肪酸氧化的影响

金勇君1，杨美子2，张凌云1，王秀云1

1.滨州医学院附属医院内分泌科，山东滨州256603； 2.滨州医学院药理学教研室，山东烟台 264003

收稿日期:2009-12-18 出版日期:2010-05-16 发布日期:2010-05-16
作者简介:金勇君（1972- ），男，副教授，医学博士，主要从事肥胖和骨质疏松研究。 Email:edrjin@yahoo.com.cn
基金资助:
国家自然科学基金资助项目（30660070）；山东省教育厅科技计划资助项目（J07YD21）

Effect of α-MSH on fatty acid oxidation in mouse skeletal muscle

JIN Yongjun1，YANG Meizi2，ZHANG Lingyun1，WANG Xiuyun1

1. Department of Endocrinology，Affiliated Hospital of Binzhou Medical University，Binzhou 256603, Shandong，China；
2. Department of Pharmacology，Binzhou Medical University，Yantai 264003, Shandong, China

Received:2009-12-18 Online:2010-05-16 Published:2010-05-16

摘要/Abstract

摘要：

目的观察α-促黑素（α-MSH）对小鼠骨骼肌细胞脂肪酸氧化的影响及其机制。方法 8周龄C57BL/6J小鼠分成生理盐水对照组和实验组（α-MSH组），两组分别皮下注射生理盐水和α-MSH后0、0.5、1、3、5h测定比目鱼肌的脂肪酸氧化限速酶-肉毒碱棕榈酰转移酶-1（CPT-1）活性。体外培养的原代骨骼肌细胞和C2C12细胞，利用RT-PCR法测定黑皮质素受体（MCR）的表达，并α-MSH处理2h后观察脂肪酸氧化。结果 α-MSH皮下注射后1、3h时CPT-1活性显著增加，与对照组相比差异有统计学意义（P＜0.05）。原代骨骼肌细胞和C2C12细胞中表达MC1R、MC3R、MC4R和MC5R，α-MSH剂量依赖性地促进脂肪酸氧化。结论 α-MSH可能通过MCR促进骨骼肌细胞脂肪酸氧化。

关键词: 促黑素；黑皮质素受体；脂肪酸氧化

Abstract:

Objective To investigate the effect and mechanism of α-Melanocyte stimulating hormone（α-MSH）on fatty acid oxidation in skeletal muscle. Methods Saline or α-MSH were subcutaneously injected into 8-week C57BL/6J male mice , and the carnitine palmitoyl transferase-1（CPT-1） activities were measured 0, 0.5, 1, 3 and 5 hours after treatment. Melanocortin receptor(MCR) subtypes in the muscle and C2C12 cells were measured by RT-PCR, and the effect of α-MSH on palmitate oxidation was also analyzed. Results CPT-1 activities were significantly increased 1 and 3 hours after treatment(P＜0.05）. MC1R, MC3R, MC4R and MC5R were expressed in C2C12 cells and primary cultured skeletal muscle, and α-MSH increased the palmitate oxidation in a dose-dependently way. Conclusion α-MSH might increased fatty acid oxidation in skeletal muscle through MCR.

Key words: α-MSH; Melanocortin receptor; Fatty acid oxidation

中图分类号:

R589.2

金勇君1，杨美子2，张凌云1，王秀云1. 促黑素对小鼠骨骼肌细胞脂肪酸氧化的影响[J]. 山东大学学报(医学版), 2010, 48(5): 9-13.

JIN Yongjun1，YANG Meizi2，ZHANG Lingyun1，WANG Xiuyun1. Effect of α-MSH on fatty acid oxidation in mouse skeletal muscle[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(5): 9-13.

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