关键词: 糖尿病；牙周炎；清热滋阴活血；细胞因子；白细胞介素1β；大鼠,Wistar

Abstract:

Objective  To observe the effect of Replenishing yin, Clearing heat and Promoting blood circulating Compound prescription on the rats with diabetic periodontitis and to explore its mechanism. Methods  10-week-old healthy male Wistar rats were randomly divided into the blank control group (group A), the diabetic periodontitis group (group B) and the traditional Chinese medicine treatment group (group C). After diabetic periodontitis models were established in group B and C , group C was fed with equivalent dosage of Honeysuckle, North Adenophora and Chuanxiong compound decoction, and group A and B were fed with an equal volume of normal saline. 2 months later, under anesthesia, boxes of bilateral maxillary including experimental zone molar were intercepted. HE staining was used to detect the general condtions and pathological changs by counting the number of infiltrating inflammatory cells in the gingival connective tissue and epithelial tissue and measuring the distance from mesial alveolar crest to the cemento-enamel junction of the experimental tooth. The venous blood was drawn out from the main vein of abdomen. After preparing serum, the level of IL-1β was measured by ELISA and the data were statistically analysed by SPSS10.0 software package.Results  The number ofinfiltrating inflammatory cells in the gingival tissue and the distance from alveolar crest to the cemento-enamel junction, were increased in groups B and C compared with those in group A, and there were significant differences among the three groups(P<0.05) . Level of IL1β in peripheral blood of group B was higher than those of groups A and C, and there was a significant difference(P＜0.05). Conclusion  Replenishing yin, Clearing heat and Promoting blood circulating Compound prescription has curative effect on diabetic periodontitis.

Key words:  Diabetes; Periodontitis; Replenishing yin,Clearing heat and Promoting blood circulating ; Cell factor; Interleukin1β; Rats，Wistar