SKOV3冻融抗原负载的DC-CIK细胞对SKOV3的杀伤作用

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (2): 131-135.

SKOV3冻融抗原负载的DC-CIK细胞对SKOV3的杀伤作用

崔莹莹1,2，周婷2，张蓓1,2，荣风年2

山东大学 1. 医学院，济南 250012； 2. 附属千佛山医院妇产科，济南 250014

收稿日期:2009-10-24 出版日期:2010-02-16 发布日期:2010-02-16
通讯作者: 荣风年(1960- ),女,主任医师、主要从事妇科肿瘤的研究。
作者简介:崔莹莹(1983- ),女,硕士研究生,主要从事妇科肿瘤的研究
基金资助:
山东省自然科学基金资助课题（Y2008C158）

Effect of DC-CIK pulsed with tumor lysate antigens of SKOV3 on cytotoxic activity against SKOV3

CUI Yingying1,2，　ZHOU Ting2，　ZHANG Bei1,2，　RONG Fengnian2

1. School of Medicine, Shandong University, Jinan 250012, China;
2.Department of Gynecology and Obstetrics, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, China

Received:2009-10-24 Online:2010-02-16 Published:2010-02-16

摘要/Abstract

摘要：

目的探讨负载人卵巢癌细胞株SKOV3冻融抗原（Ag）的树突状细胞（DC）与细胞因子诱导的杀伤细胞（CIK）共培养后对SKOV3杀伤作用的影响。方法取12例卵巢癌患者外周血，常规诱导出DC及CIK细胞，以SKOV3冻融Ag负载DC，经Ag负载与未经Ag负载的DC分别和CIK细胞共培养，流式细胞技术分析DC、AgDC、AgDCCIK 3组DC细胞表型，CIK、DCCIK、AgDCCIK组CIK细胞表型；乳酸脱氢酶释放法测CIK组、DC-CIK组、Ag-DC-CIK组对SKOV3杀伤活性。结果 Ag-DC-CIK组中DC细胞成熟表型高于DC及Ag-DC组(P＜0.01)；Ag-DC-CIK组中CIK细胞成熟表型高于CIK及DC-CIK组(P＜0.01)；Ag-DC-CIK组对SKOV3杀伤率高于CIK及DC-CIK组(P＜0.01)。结论负载SKOV3冻融Ag的DC与CIK共培养可促进DC、CIK的成熟,且可提高对SKOV3的杀伤作用。

关键词: 树突状细胞；细胞因子诱导的杀伤细胞；抗原

Abstract:

Objective To investigate the effect of dendritic cells(DC) pulsed with tumor lysate antigens of human ovarian cancer cell line SKOV3 co-cultured with cytokine induced killer(CIK) cells on cytotoxic activity against SKOV3. Methods Blood isolated from 12 patients with ovarian cancer was separately induced to obtain CIK and DC. Unstimulated DC and DC pulsed with tumor lysate antigens of SKOV3 were individually co-cultured with CIK. The surface molecule expressions of DC in the DC group, Ag-DC group and Ag-DC-CIK group, and the surface molecule expressions of CIK in the CIK group, DC-CIK group and Ag-DC-CIK group were measured by flow cytometry. The cytotoxic activities against SKOV3 of the CIK group, DC-CIK group and Ag-DC-CIK group were measured using lactate dehydrogenase(LDH) assay. Results Compared with the DC group and the Ag-DC group, the Ag-DC-CIK group had a significant increase of mature phenotypes of DC(P＜0.01); the mature phenotypes of CIK in the Ag-DC-CIK group also increased(P＜0.01); the cytotoxic activity against SKOV3 cells of the Ag-DC-CIK group was much higher than that of the CIK group and the DC-CIK group(P＜0.01). Conclusion DC pulsed with tumor lysate antigens of SKOV3 co-cultured with CIK cells can promote the mutation of both DC and CIK and improve cytotoxic activity against SKOV3.

Key words: Dendritic cells; Cytokine induced killer cells; Antigen

中图分类号:

R737.31

崔莹莹1,2，周婷2，张蓓1,2，荣风年2. SKOV3冻融抗原负载的DC-CIK细胞对SKOV3的杀伤作用[J]. 山东大学学报(医学版), 2010, 48(2): 131-135.

CUI Yingying1,2，　ZHOU Ting2，　ZHANG Bei1,2，　RONG Fengnian2. Effect of DC-CIK pulsed with tumor lysate antigens of SKOV3 on cytotoxic activity against SKOV3[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(2): 131-135.

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