下咽癌中maspin表达与血管生成和淋巴管生成的相关性

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (12): 82-.

下咽癌中maspin表达与血管生成和淋巴管生成的相关性

邹纪东1，徐伟1，吕正华1，曹洪源1，王海波1，栾信庸2

山东大学 1.附属省立医院耳鼻咽喉头颈外科，济南 250021；
2. 齐鲁医院耳鼻咽喉头颈外科，济南 250012

收稿日期:2010-09-29 发布日期:2010-12-16
通讯作者: 徐伟（1967- ），男，主任医师，主要从事头颈肿瘤基础与临床研究。 E-mail: xwhns@yahoo.com.cn
作者简介:邹纪东（1976），男，主治医师，主要从事头颈肿瘤基础与临床研究。 E-mail:zjdent@126.com

Correlation of maspin with angiogenesis and lymphangiogenesis in hypopharyngeal carcinoma

ZOU Ji-dong1, X Wei1, L Zheng-hua1, CAO Hong-yuan1, WANG Hai-bo1, LUAN Xin-yong2

Shandong University 1. Department of Otorhinolaryngology Head and Neck Surgery,
Provincial Hospital, Jinan 250021, China;
2. Department of Otorhinolaryngology Head and Neck Surgery, Qilu Hospital, Jinan 250012, China

Received:2010-09-29 Published:2010-12-16

摘要/Abstract

摘要：

目的观察下咽癌中maspin蛋白的表达，探讨maspin在下咽癌发生发展中的作用及其作为预后指标的意义。并探讨maspin与血管生成和淋巴管生成的关系。方法应用免疫组化法（SP法）对53例下咽癌组织进行maspin染色，分析maspin表达与下咽癌的组织学类型、临床的分期、淋巴结转移和预后等病理特征的关系。分别用CD105和LYVE-1标记新生血管和新生淋巴管，分析maspin表达与微血管密度（MVD）和微淋巴管密度（LVD）的关系。结果 maspin在下咽癌中的阳性率为47.2%(25/53)，在不同分化程度和有无淋巴结转移的下咽癌中表达差异有统计学意义(P均<0.05)。maspin表达阳性者中位生存时间(67个月)与表达阴性者(36个月)差异有统计学意义(P<0.05)。在maspin阳性表达的25例下咽癌中，MVD均值为6.56±2.22，LVD均值为10.76±3.37，maspin阴性表达的28例中，MVD均值为8.14±2.46，LVD均值为15.04±4.57。t检验显示，maspin在下咽癌中的表达与MVD和LVD呈负相关，差异有统计学意义(P均<0.05)。结论 maspin表达降低与下咽癌病理分化程度低、淋巴结转移有关。maspin可能对下咽癌中的血管生成及淋巴管生成起抑制作用。

关键词: 下咽癌；maspin；微血管密度；微淋巴管密度

Abstract:

Objective To detect the expression of maspin in hypopharyngeal carcinoma and its roles in the development of cancer. And to explore the correlation of maspin with angiogenesis and lymphangiogenesis. Method The expression of maspin protein was examined in 53 patients with hypopharyngeal squamous cell carcinoma by immunohistochemical staining. Microvessel density (MVD) and lymphatic vessel density (LVD) were counted in endothelial cells immunostained with anti-CD105 monoclonal antibody and anti-LYVE-1 multiclone antibody. The correlations of maspin with clinicopathological features, LVD, MVD were analyzed. Result Maspin were found in in 47.2%(25/53) hypopharyngeal carcinoma cases. There were significances among different cell differentiations and between the tumors with or without lymphatic metastasis (P<0.05). The median survival time of positive maspin cases(67 months) was significantly longer then the negative(36 months). In 25 positive cases, the mean MVD was 6.56±2.22 and the mean LVD was 10.76±3.37. As for the 28 negative cases, the mean MVD was 8.14±2.46, and the mean LVD was 15.04±4.57.In the t test analysis, the expression of maspin was negatively correlated with MVD and LVD (P<0.05). Conclusion The reduction of maspin′s expression is correlated with poor differentiation and lymphatic metastasis. In hypopharyngeal carcinoma, maspin may inhibit angiogenesis and lymphangiogenesis.

Key words: Hypopharyngeal carcinoma; maspin; MVD; LVD

中图分类号:

R739.63

邹纪东1，徐伟1，吕正华1，曹洪源1，王海波1，栾信庸2. 下咽癌中maspin表达与血管生成和淋巴管生成的相关性[J]. 山东大学学报(医学版), 2010, 48(12): 82-.

ZOU Ji-dong1, X Wei1, L Zheng-hua1, CAO Hong-yuan1, WANG Hai-bo1, LUAN Xin-yong2 . Correlation of maspin with angiogenesis and lymphangiogenesis in hypopharyngeal carcinoma[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(12): 82-.

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