关键词: 大蒜油；环磷酰胺；H22；化疗；小鼠

Abstract:

Objective      To observe the preventive effect of Garlic oil（GO） on H22 tumorbearing mice in conjuction with cyclophosphamide（CP） chemotherapy. Methods     60 mice were randomized into four groups: tumor model, CP group, 25μg/g GO +CP and 50μg/g GO+CP group.  Each mouse was inoculated with Hepatoma 22 cells subcutaneously at the right armpit, followed by 13 days of GO administration. Mice were sacrificed by cervical dislocation, and then tumor mass and spleens were dissected and weighted. In addition, WBC, spleen index, spleen nodes count, the contents of DNA and Nucleated marrow cells (NMC) in bone marrow, bone marrow micronucleus were detected. Results     Compared with the tumor model, the tumor mass of CP group  decreased by 60.5%, while WBC, spleen index, spleen nodes count, the contents of DNA in bone marrow and NMC were lowered by 65.9%, 34.8%，63.1%，50.1%，64.3%（P<0.05）, respectively. Compared with the CP group, 50μg/g GO caused a tumorinhibiting rate of 62.0%(P>0.05), and increased WBC, spleen index, spleen nodes count, the content of DNA and NMC（P<0.05）. Conclusions     Garlic oil can attenuate the leukopenia induced by CP on tumor-bearing mice without influencing its anti-tumor activity.

Key words: Garlic oil; Cyclophosphamide; H22; Chemotherapy; Mouse