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山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (12): 124-.

• 论文 • 上一篇    下一篇

胱抑素C对人动脉平滑肌细胞中组织蛋白酶S和细胞外基质的影响

王群1, 杜贻萌2, 董兆强2, 刘玉胜2, 鹿庆华2   

  1. 山东大学 1.南区医院, 济南 250061;  2.第二医院心内科,  济南 250033
  • 收稿日期:2010-10-27 发布日期:2010-12-16
  • 通讯作者: 鹿庆华（1964- ），男，教授，主要研究方向为临床冠脉介入治疗及术后再狭窄基础及临床干预。E-mail:lqhzhy@163.com
  • 作者简介:王群（1971- ），女，主治医师，硕士，主要研究方向为临床冠脉介入治疗及术后再狭窄基础及临床干预。

Effect of Cystatin C on Cathepsin S and extracellular matrix  in human arterial smooth muscle cells

WANG Qun1, DU Yi-meng2, DONG Zhao-qiang2, LIU Yu-sheng2, LU Qing-hua2   

  1. 1. Hospital of South Division, Shandong University, Jinan 250061, China；2.Department of Cardiovascular Diseases, The Second Hospital of Shandong University, Jinan 250033, China
  • Received:2010-10-27 Published:2010-12-16

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摘要：

目的     研究胱抑素C(CysC)对人动脉平滑肌细胞(ASMC)中组织蛋白酶S (CatS)和细胞外基质的影响。方法      以正常ASMC为空白对照组, RT-PCR及Western-blot法测定CysC高表达质粒转染(CysC高表达组)及小干扰RNA(siRNA)分别干扰CysC (干扰组) 0、12、24、48、72h后CatS的表达；ELISA法测定空白对照组、CysC高表达24h组和48h组、干扰48h组和72h组中Ⅰ型胶原、层粘连蛋白(LN)和纤连蛋白(FN)的含量。结果      与空白对照组比较，CysC高表达组中CatS的表达较少，差异无统计学意义(P>0.05)；随干扰时间延长，干扰组中CatS的表达明显增强(P<0.01)；Ⅰ型胶原含量在各组虽有轻微增加或减少，但差异均无统计学意义(P>0.05)；LN和FN含量在CysC高表达24h组和48h组均有不同程度增加，LN含量在干扰72h组明显减少，FN含量在干扰48h组和72h组明显减少(P均< 0.05)。 结论      正常ASMC中CatS表达极少；CysC和CatS在ASMC中的表达呈负相关；CysC和CatS的平衡关系对层粘连蛋白和纤连蛋白均有不同程度的影响，但对Ⅰ型胶原的影响不明显。

关键词: 胱抑素C；组织蛋白酶S；细胞外基质；人动脉平滑肌细胞

Abstract:

Objective     To study the effect of Cystatin C on Cathepsin S and extracellular matrix (ECM) in human arterial smooth muscle cells(ASMCs). Methods     ASMCs were divided into the control group(normal cells), the Cystatin C highly expressed group, the Cystatin C silenced group and the independent interference group. Expression of Cathepsin S was detected by RT-PCR and Western blot at different time. Contents of collagenⅠ, laminin(LN) and fibronectin(FN) were detected by ELISA in ASMCs of the control group, the Cystatin C highly expressed group(24h and 48h) and the Cystatin C silenced group(48h and 72h). Results     Expression of Cathepsin S in the Cystatin C highly expressed group was lower than in the control group, and the difference between the two groups was not statistically significant（P>0.05）. Expression of Cathepsin S in the Cystatin C silenced group was obviously strengthened with the increase of interference time(P<0.01). While at the same time, expression of Cystatin C was weakened. The result of ELISA showed that content of collagenⅠ in each group was increased or decreased, while the differences were not statistically significant（P>0.05）. Contents ofLN and FN increased in the Cystatin C highly expressed group (24h and 48h), and the differences were both statistically significant compared with the controls(P<0.05). Compared with the control group, content of LN in Cystatin C silenced group (48h) was insignificantly decreased（P>0.05）, while it was obviously decreased in Cystatin C silenced group (72h)（P<0.05）.Compared with the control group, contents of FN in Cystatin C silenced group (48h and 72h) decreased markedly, and the differences were both statistically significant（P<0.05）. Conclusion      Expression of Cathepsin S is very low in normal ASMCs. Expressions of Cystatin C and Cathepsin S in ASMCs are inversely correlated. The balance of Cystatin C and Cathepsin S has a significant effect on LN and FN to different degrees, but no significant effect on collagenⅠ.

Key words: Cystatin C; Cathepsin S; Extracellular matrix; Human arterial smooth muscle cells

中图分类号: 

  • R541.4
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