济南市售小米和玉米中赭曲霉素A污染状况研究

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (11): 125-128.

济南市售小米和玉米中赭曲霉素A污染状况研究

杨延友1，高文花2，温红玲3，蒲双双3，宋艳艳3，许洪芝3，赵丽3，李凤琴4

1.山东大学公共卫生学院，济南 250012； 2.山东大学校医院，济南 250100；
3. 山东大学公共卫生学院微生物检验所，济南 250012；
4. 中国疾病预防控制中心营养与食品安全所，北京 100052

收稿日期:2010-09-15 出版日期:2010-11-16 发布日期:2010-11-16
通讯作者: 赵丽（1965- ），女，副教授，博士，主要从事微生物检验。 E-mail：dlzhl@sdu.edu.cn
作者简介:杨延友（1990- ）,男，主要从事微生物检验。
基金资助:
国家高技术研究发展计划（863计划）资助项目（2007AA10Z423）。

Contamination of ochratoxin A in millet and maize sold in Jinan

YANG Yan-you1， GAO Wen-hua2， WEN Hong-ling3, PU Shuang-shuang3, SONG Yan-yan3, XU Hong-zhi3, ZHAO Li3, LI Feng-qin4

1. School of Public Health, Shandong University， Jinan 250012， China；
2. School Hospital，Shandong University, Jinan 250100, China；
3. Institute of Microbiology Examination, School of Public Health, Shandong University， Jinan 250012, China；
4. Institute of Nutrition and Food Safety， Chinese Center for Disease Control and Prevention， Beijing 100052, China

Received:2010-09-15 Online:2010-11-16 Published:2010-11-16

摘要/Abstract

摘要：

目的了解济南市售小米和玉米中赭曲霉毒素A的污染情况。方法采用竞争抑制酶联免疫吸附测定（ELISA）法检测小米和玉米中赭曲霉毒素A的提取溶剂、检测条件、回收率等，并对其进行优化研究，并利用优化出的条件对济南市5个区市售小米和玉米中赭曲霉毒素A含量进行检测并评价。结果含有5%氯化钠的60%甲醇水溶液对谷物中赭曲霉毒素A的提取效果较为理想，检测的所有样品中，小米、玉米赭曲霉毒素A检出率分别为41%和38%,其中，超市小米赭曲霉毒素A检出率（56%）明显高于集市小米（26%）(P＜0.05)。赭曲霉毒素A污染水平在0.40～4.45μg/kg之间，中位数为1.67μg/kg, 集市和超市出售的小米、玉米中赭曲霉毒素A污染水平均无统计学意义(P＞0.05)。本次检测所有样品中的赭曲霉毒素A含量均未超过国家规定的粮食中赭曲霉毒素A的限量标准。结论济南市售的小米和玉米中均不同程度地存在赭曲霉毒素A污染，但污染水平均未超过国家标准。

关键词: 赭曲霉毒素A；小米；玉米；酶联免疫吸附试验

Abstract:

Objective To determine the contamination of ochratoxin A in millet and maize commercially available in Jinan. Methods An optimization study of the extraction solvent, testing conditions and recovery rates in detection of ochratoxin A in millet and maize by ELISA was carried out. Millet and maize samples collected from 5 districts of Jinan were analyzed under optimum conditions. Results Optimal ochratoxin A extraction was obtained when the extracting agent contained 5% sodium chloride and 60% methanol. For all the samples, the ochratoxin A detectable rate in millet was 41%, while in maize it was 38%. The ochratoxin A detectable rate in millet from supermarkets was 56%, which was significantly higher than that in the millet samples from markets (26%) (P＜0.05). The ochratoxin A concentration level ranged between 0.40 and 4.45μg/kg, and the median was 1.67μg/kg. No significant difference in the ochratoxin A pollution level between millet and maize was found (P>0.05). Ochratoxin A contents in all samples were below the tolerance limit set by the state for ochratoxin A in grain. Conclusions The millet and maize commercially available in Jinan are contaminated by ochratoxin A to some extent, but the ochratoxin A content in the detected samples is below the tolerance limit.

Key words: Ochratoxin A； Millet； Maize； Enzyme-linked immunosorbent assay

中图分类号:

R117

杨延友1，高文花2，温红玲3，蒲双双3，宋艳艳3，许洪芝3，赵丽3，李凤琴4. 济南市售小米和玉米中赭曲霉素A污染状况研究[J]. 山东大学学报(医学版), 2010, 48(11): 125-128.

YANG Yan-you1， GAO Wen-hua2， WEN Hong-ling3, PU Shuang-shuang3, SONG Yan-yan3, XU Hong-zhi3, ZHAO Li3, LI Feng-qin4 . Contamination of ochratoxin A in millet and maize sold in Jinan[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(11): 125-128.

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