关键词: 哮喘；多态性，单核苷酸；裂解素-金属蛋白酶33基因；等位基因；聚合酶链反应, 药敏试验; 抗药性; 耐药性; Breakpoint

Abstract:

Objective    To investigate the association between polymorphism of the F+1 locus allele in the ADAM33 gene and bronchial asthma. Methods    Allele-specific polymerase chain reaction (AS-PCR) and DNA sequencing were used to determine polymorphism of the F+1 locus allele in the ADAM33 gene in 126 unrelated patients with bronchial asthma and 121 unrelated healthy controls. Results     Genotype distributions of the F+1 locus allele in the ADAM33 gene in the patient group and the control group did not deviate from the HardyWeinburg equilibrium. Frequencies of the genotypes (CC,CT,TT) were 54.0% (n=68), 38.1% (n=48) and 7.9% (n=10) in the 126 asthmatic patients and 57.9% (n=70), 38.0% (n=46) and 4.1% (n=5) in the 121 controls, respectively. There was no significant difference in distribution of the genotypes (CC,  CT, TT) between asthmatic patients and healthy controls (χ2=1.638, P＞0.05). Frequencies of the alleles (C, T) were 0.730 and 0.270 in asthmatic patients and 0.769 and 0.231 in the controls, respectively, and there was no significant difference in allele frequency between them (χ2 =0.970,P＞0.05). Conclusion      Polymorphism of the F+1 locus allele in theADAM33 gene(CC,CT,TT) exists in asthmatic patients, but the polymorphism is not significantly associated with the susceptibility to bronchial asthma.

Key words: Asthma; Polymorphism， single; ADAM 33 gene； Alleles； Polymerase chain reaction, Antimicrobial susceptibility testing； Antimicrobial resistance； Antimicrobial tolerance； Breakpoint