肺癌的胆碱显像与胆碱代谢的变化

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (1): 123-.

肺癌的胆碱显像与胆碱代谢的变化

芮军，黄召勤，李昕，彭忠民，刘庆伟

山东大学附属省立医院医学影像科, 济南 250021

收稿日期:2009-09-09 出版日期:2010-01-16 发布日期:2010-01-16
通讯作者: 刘庆伟(1958- ),男，教授，博士，主要从事肿瘤性病变的早期解剖和功能影像综合评价研究
作者简介:芮军（1974- ），男，硕士研究生，主要从事肺部疾病的影像学研究。 E-mail:annirj@163.com

C-choline PET/CT imaging and the change of choline metabolism in lung cancer

RUI Jun, HUANG Zhaoqin, LI Xin, PENG Zhongmin, LIU Qingwei

Department of Radiology, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China

Received:2009-09-09 Online:2010-01-16 Published:2010-01-16

摘要/Abstract

摘要：

目的探讨肺癌的胆碱显像与肺癌组织细胞中胆碱代谢的变化。方法选取经手术病理证实的18例肺癌患者，术前均进行11-C-胆碱PET/CT显像，用目测法和半定量法分析显像结果,术后将取得的肺癌组织及正常肺组织用RT-PCR和Western blot方法，检测胆碱乙酰基转移酶(ChAT)和胆碱激酶（Chok）基因的表达,分析肺癌的胆碱显像与肺癌组织细胞中胆碱代谢的变化。结果 18例肺癌的PET/CT图像均显示胆碱放射性摄取明显增高,18例肺癌组织细胞中，14例Chok mRNA及蛋白质的表达比正常肺组织升高，9例肺癌组织细胞ChAT mRNA及蛋白质表达升高，其中8例Chok及ChAT表达均升高。鳞癌与腺癌对胆碱的平均标准摄取值（SUV）差异无统计学意义。结论肺癌的胆碱显像有异常增高，其增高原因与肺癌细胞中胆碱代谢的磷酸化途径及乙酰化途径增高有关。

关键词: 肺肿瘤；胆碱O-乙酰基转移酶；胆碱激酶；体层摄影术,发射型计算机；胆碱

Abstract:

Objective To investigate the 11-C-choline PET/CT imaging of lung cancer and the change of choline metabolism in the cells of lung cancer. Methods 11-C-choline PET/CT imaging was performed in 18 patients with a lung tumor confirmed by operation and pathology. RT-PCR and Western blot were used to investigate expressions of choline acetyltransferase (ChAT) and choline kinase (ChoK) genes in lung cancer specimens and normal lung tissues. Results The uptake of 11-C-choline was increased in all the lesions. Expressions of ChAT mRNA and protein were enhanced in 9 of the 18 lung tumor specimens; and expressions of ChoK mRNA and protein were increased in 14 of 18 lung tumor specimens compared with those of normal lung tissues. 8 of 18 lung cancer specimens were obvious increased in expressions of both ChoK and ChAT. The difference was not statistically significant between squamous cell carcinoma and adenocarcinoma inSUV value. Conclusion There is abnormally high uptake of 11-C-choline in a lung tumor, and the activities of both ChAT and ChoK are increased in cells of lung cancer.

Key words: Lung neoplasms; Choline O-acetyltransferase; Choline kinase; Tomography, emission-computed; Choline

中图分类号:

R817.4

芮军，黄召勤，李昕，彭忠民，刘庆伟. 肺癌的胆碱显像与胆碱代谢的变化[J]. 山东大学学报(医学版), 2010, 48(1): 123-.

RUI Jun, HUANG Zhaoqin, LI Xin, PENG Zhongmin, LIU Qingwei. C-choline PET/CT imaging and the change of choline metabolism in lung cancer[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(1): 123-.

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