DcR3蛋白和caspase3在结直肠癌和癌前病变中的表达及其意义

山东大学学报(医学版) ›› 2009, Vol. 47 ›› Issue (8): 79-84.

DcR3蛋白和caspase3在结直肠癌和癌前病变中的表达及其意义

张世栋¹，郝艳萍¹，胡顺明¹，于成功¹，周强²

南京大学医学院附属鼓楼医院 1．消化科；2．病理科，南京 210008

收稿日期:2009-03-09 发布日期:2009-08-16
通讯作者: 于成功(1965- )，男，博士，教授，主要从事结直肠癌和炎症性肠病研究。 Email：chenggong.yu＠gmail.com
作者简介:张世栋(1980- )，男，硕士研究生，主要从事结直肠癌的早期诊断研究。 Email：ndzsd520@gmail.com
基金资助:
南京市社会发展科技计划项目资助课题（No. 200701111）

Expressions of DcR3 and caspase3 and their significance in colorectal adenocarcinoma and precancerous lesions

ZHANG Shidong ¹，　HAO Yanping ¹，　HU Shunming ¹，　YU Chenggong ¹，　ZHOU Qiang ²

1．Departments of Gastroenterology； 2．Departments of Pathology， Affiliated Drum Tower Hospital of Medical School of Nanjing University， Nanjing 210008， China

Received:2009-03-09 Published:2009-08-16

摘要/Abstract

摘要：

目的探讨诱捕受体3(DcR3)蛋白和caspase3在结直肠癌癌变过程中的表达及其与结直肠癌临床病理特征之间的关系。方法采用免疫组织化学SP法检测70例结直肠癌组织，59例结直肠腺瘤（腺瘤伴低度上皮内瘤变37例，腺瘤伴高度上皮内瘤变22例）和10例结直肠正常粘膜组织中DcR3和caspase3蛋白的表达，分析其与临床病理特征之间的关系。结果①DcR3 在结直肠正常粘膜、腺瘤、结直肠癌组织中的阳性表达率分别为0（0/10）、39.0%（23/59）和70%(49/70) ，而caspase3阳性表达率分别为100% (10/10)、33.9%(20/59)、28.6%(20/70)；②在结直肠癌组织中DcR3和caspase3的表达与患者性别、年龄、肿瘤部位、组织类型、分化程度等均无相关性(P>0.05)，但DcR3的表达与Dukes分期有明显的相关性，Dukes C/D期者，DcR3阳性表达率低（P<0.05)；③在结直肠腺瘤组织中caspase3的表达与腺瘤分化程度的比较差异有统计学意义（P<0.05)，而DcR3和caspase3的表达与患者的年龄、性别、腺瘤部位、大小、数目、表面形态等差异均无统计学意义（P>0.05）。结论①DcR3和caspase3可能共同参与了结直肠癌的发生发展；②检测DcR3和caspase3的表达对于结直肠癌的早期诊断可能有一定的价值。

关键词: 结直肠肿瘤；诱捕受体3；半胱氨酸天冬氨酸蛋白酶3；免疫组织化学

Abstract:

To investigate the relationship between expressions of decoy receptor3 (DcR3) and caspase3 and clinicopathological parameters in colorectal carcinogenesis. MethodsExpressions of DcR3 and caspase3 were determined by immunohistochemical SP staining in a series of 70 colorectal cancer (CRC)，59 colorectal adenoma (CRA，including 22 highgrade colorectal intraepithelial neoplasia and 37 lowgrade colorectal intraepithelial neoplasia) and 10 normal colorectal mucosa (NCM)．Multiple clinicopathological factors were analyzed based on their relation to expressions of DcR3 and caspase3． Results① Expressive rate of DcR3 in NCM，CRA and CRC was 0（0/10），39.0%（23/59）and 70%(49/70)　respectively．However，expressive rate of caspase3 in the three groups was 100.0% (10/10)，33.9% (20/39) and 28.6% (20/50),　respectively．② Expressions of DcR3 and caspase3 in CRC were not associated with sex， age， tumor location，differentiation and histopathological type (P>0.05)， but expression of DcR3 in CRC with Dukes C/D was significantly lower than that of Dukes A/B (P<0.05)．③ Expression of caspase3 in CRA was significantly correlated with differentiation status(P<0.05)，but there was no significant difference between expressions of DcR3 and caspase3 and other clinicopathological features such as sex， age， tumor location，size， number, and morphological characteristics(P>0.05)． Conclusion① Both DcR3 and caspase3 may be involved in the pathogenesis of colorectal cancer and play an important role in the development of colorectal cancer in Chinese people． ② Detecting expressions of DcR3 and caspase3 may be useful in the early diagnosis of colorectal cancer．

Key words: Colorectal neoplasms； DcR3； Caspase3； Immunohistochemistry

中图分类号:

R735.3+5

. DcR3蛋白和caspase3在结直肠癌和癌前病变中的表达及其意义[J]. 山东大学学报(医学版), 2009, 47(8): 79-84.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed