CYP2E1、GSTT1基因多态性与非霍奇金淋巴瘤易感性的关系

山东大学学报(医学版) ›› 2009, Vol. 47 ›› Issue (7): 57-60.

CYP2E1、GSTT1基因多态性与非霍奇金淋巴瘤易感性的关系

宋宝¹，刘杰²，郑劲松³，宋现让¹，吕丽燕¹，石艳⁴

1. 山东省肿瘤医院基础研究中心，济南 250117； 2. 山东省肿瘤医院内三科，济南 250117；
3. 山东省肿瘤医院PET室，济南 250117； 4. 山东大学齐鲁医院血液科，济南 250012

收稿日期:2008-09-24 发布日期:2009-07-16
通讯作者: 刘杰（1969- ），男，主治医师，主要从事肿瘤分子生物学研究。Email:lj691012@126.com
作者简介:宋宝（1973- ），女，硕士，助理研究员，主要从事肿瘤分子生物学研究。

Relationship between cytochrome P450 enzyme 2E1，glutathione Stransferase theta 1 genetic polymorphisms and nonHodgkin lymphoma susceptibility

SONG Bao ¹, LIU Jie ², ZHENG Jinsong ³, SONG Xianrang ¹, L Liyan ^1, SHI Yan ⁴

. Department of Basic Research Center, Shandong Tumor Hospital, Jinan 250117, China；
2. Third Department of Internal Medicine, Shandong Tumor Hospital, Jinan 250117, China；
3. Department of PET, Shandong Tumor Hospital, Jinan 250117, China；
4. Department of Hemotology, Qilu Hospital of Shandong University, Jinan 250012, China

Received:2008-09-24 Published:2009-07-16

摘要/Abstract

摘要：

目的探讨细胞色素P450酶2E1(CYP2E1)和谷胱甘肽硫转移酶(GSTT1)基因多态性与非霍奇金淋巴瘤（NHL）易感性的关系。方法分别采用聚合酶链反应-限制性片段长度多态性技术和聚合酶链反应技术对254例NHL患者和254例健康对照者CYP2E1和GSTT1基因多态性进行分析。结果NHL组和对照组CYP2E1基因多态性分布无明显差异(P>0.05)；NHL组GSTT1基因缺失型频率明显高于对照组（48.0% vs 37.8%，P<0.05）。对NHL亚型分析显示，与GSTT1野生型者相比，携带GSTT1缺失型者发生弥漫大B细胞性淋巴瘤和滤泡性淋巴瘤风险分别为1.78倍（95%CI：1.12～2.82，P=0.02）和1.69倍（95%CI：1.03～2.77，P=0.04）。基因多态联合作用分析显示，CYP2E1野生型伴GSTT1缺失型者发生NHL的风险为1.83倍(95%CI：1.14～2.95，P=0.01)。结论GSTT1基因多态性可能是NHL的独立易感因素，GSTT1缺失型与CYP2E1野生型联合作用可进一步增加NHL的发病风险。

关键词: 非霍奇金淋巴瘤；基因多态性；细胞色素P450酶；谷胱甘肽硫转移酶

Abstract:

To explore the relationship between cytochrome P450 enzyme 2E1(CYP2E1), glutathione Stransferase theta 1(GSTT1) genetic polymorphism and individual susceptibility to nonHodgkin lymphoma (NHL). MethodsCYP2E1 genotypes were detected by the polymerase chain reactionrestriction fragment length polymorphism（PCRRFLP） technique in 254 patients with NHL and 254 healthy controls. GSTT1 genotypes were detected with the polymerase chain reaction（PCR）　technique in the two groups. ResultsCYP2E1 gene polymorphisms were not statistically different between controls and patients with NHL(P>0.05). Frequencies of GSTT1 null genotypes were significantly increased in the NHL group compared with the control group (48.0% vs 37.8%, P<0.05). In subtype analysis, individuals carrying the GSTT1 null genotype had a 1.78fold risk of diffuse large Bcell lymphoma(DLBCL) (95% CI: 1.122.82, P=0.02) and a 1.69fold risk of follicular lymphoma (FL)（95%CI: 1.032.77, P=0.04）compared with the wild genotype. Combined genotype analysis showed that individuals carrying the CYP2E1wild genotype and GSTT1 null genotype had a 1.83fold risk of NHL(95%CI:1.14  2.95，P=0.01). ConclusionGSTT1 polymorphism may be an independent risk factor of NHL. CYP2E1 wildtype in combination with GSTT1 nulltype further increases the incidence of NHL.

Key words: NonHodgkin lymphoma; Gene polymorphism; Cytochrome P450 enzyme; Glutathione Stransferase

中图分类号:

R730.231

. CYP2E1、GSTT1基因多态性与非霍奇金淋巴瘤易感性的关系[J]. 山东大学学报(医学版), 2009, 47(7): 57-60.

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