RNA干扰抑制视网膜母细胞瘤细胞血管内皮生长因子的表达

山东大学学报(医学版) ›› 2009, Vol. 47 ›› Issue (7): 13-15.

RNA干扰抑制视网膜母细胞瘤细胞血管内皮生长因子的表达

刘德荣¹，蔡可丽¹, 卓建¹，吴晓莉³，杨雪莉¹，杨博²

1. 山东大学齐鲁医院眼科，济南 250012； 2. 山东大学齐鲁医院麻醉科，济南 250012；
3. 山东省荣军总医院眼科，济南 250013

收稿日期:2008-12-24 发布日期:2009-07-16
通讯作者: 蔡可丽（1961- ），女，教授，主要从事白内障和眼肿瘤研究。Email: ckl@jn.gov.cn
作者简介:刘德荣（1981- ），女，硕士研究生，主要从事白内障和眼肿瘤研究。

RNA interference inhibits VEGF expression in retinoblastoma cells: an experimental study

1. Department of Ophthalmology, Qilu Hospital of Shandong University, Jinan 250012, China;
2. Department of Anesthesiology, Qilu Hospital of Shandong University, Jinan 250012, China;
3. Department of Ophthalmology, Shandong Rongjun Hospital, Jinan 250013, China

Received:2008-12-24 Published:2009-07-16

摘要/Abstract

摘要：

目的利用RNA干扰（RNAi）技术，研究血管内皮生长因子（VEGF）编码基因的siRNA对人视网膜母细胞瘤（RB）细胞的影响，从而探讨通过抑制血管内皮生长因子在视网膜母细胞瘤组织中的表达来治疗视网膜母细胞瘤的机理。方法将VEGF编码基因特异的siRNA转染入视网膜母细胞瘤细胞，采用RTPCR和Westernblot技术检测siRNA处理前后RB细胞VEGF基因表达变化。MTT法检测siRNA处理前后RB细胞生长的抑制情况。结果RTPCR和Westernblot均显示VEGF特异性siRNA对视网膜母细胞瘤细胞血管内皮生长因子基因的表达有抑制作用，MTT法检测RB细胞生长的抑制情况显示，抑制率可达40%。结论VEGF特异性siRNA的特异性基因沉默作用对RB细胞VEGF基因的表达有抑制作用。

关键词: RNA干扰；视网膜母细胞瘤；血管内皮生长因子

Abstract:

To explore the possibility of VEGF as a therapeutic target for retinoblastoma, we employed RNA interference to inhibit VEGF expression in retinoblastoma cells. MethodsSiRNA oligo of VEGF was synthesized and transfected into retinoblastoma cells. VEGF mRNA expression and protein content were respectively determined by reverse transcription polymerase chain reaction(RTPCR) and Western blot. Retinoblastoma cell proliferation was detected by MTT reduction assay. ResultsVEGF mRNA expression and protein content were significantly decreased by VEGFtargeted siRNA. MTT reduction assay showed that the inhibition ratio was 40%. ConclusionVEGFtargeted siRNA can effectively inhibit expression of VEGF in retinoblastoma cells.

Key words: NA interference; Retinoblastoma; Vascular endothelial growth factor

中图分类号:

R739.7

. RNA干扰抑制视网膜母细胞瘤细胞血管内皮生长因子的表达[J]. 山东大学学报(医学版), 2009, 47(7): 13-15.

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