低频超声辅助尿激酶溶栓对大鼠脑梗死基质金属蛋白酶9的影响

山东大学学报(医学版) ›› 2009, Vol. 47 ›› Issue (12): 50-53.

低频超声辅助尿激酶溶栓对大鼠脑梗死基质金属蛋白酶9的影响

宋玉强^1，2，毕建忠¹

1. 山东大学第二医院神经内科，济南 250033； 2. 青岛大学医学院附属医院神经内科，山东青岛 266003

收稿日期:2009-06-16 出版日期:2009-12-16 发布日期:2009-12-16
通讯作者: 毕建忠（1962- ），男，主任医师，医学博士，博导，主要从事脑血管病基础和临床的研究。 Email: bjz@sdu.edu.cn

Effects of lowfrequency ultrasoundenhanced thrombolysis by urokinase on expression of matrix metal loproteinase9 during cerebral infarction in rats

SONG Yuqiang ^1,2 , BI Jianzhong ¹

1. Department of Neurology, Second Hospital of Shandong Univeristy， Jinan 250033, China;
2. Department of Neurology, Affiliated Hospital of Medical College of Qingdao University, Qingdao 266003, Shandong, China

Received:2009-06-16 Online:2009-12-16 Published:2009-12-16
About author:宋玉强（1970- ），男，副主任医师，博士研究生，主要从事脑血管病基础和临床的研究。 Email: doctorsyq@sina.com

摘要/Abstract

摘要：

目的观察低频超声辅助尿激酶静脉溶栓治疗大鼠急性脑梗塞后基质金属蛋白酶9(MMP9)的变化，探讨其在低频超声助溶脑缺血再灌注损伤及出血性转化中的作用。方法应用血栓栓塞法制备Wistar大鼠脑梗塞模型，分别给予尿激酶、低频超声照射，采用NSS评价治疗前后神经功能，采用红四氮唑染色法测定脑梗塞体积，采用免疫组化法监测MMP9 的表达。结果梗塞组用药前后NSS评分差异不明显（t=0.85,P>0.05）；尿激酶组、低频超声加尿激酶组给药后24?h NSS评分明显低于溶栓前（t=12.59, t=15.31,　P均<0.01）。3组梗塞灶体积有显著性差异（F=125.99, P<0.01），尿激酶组、低频超声加尿激酶组梗塞灶体积显著小于梗塞组（q=18.02,P<0.01；q=20.62,P<0.01），两组之间差异不显著（q=2.59，P>0.05）。尿激酶组和低频超声加尿激酶组MMP9表达阳性率及光密度值均显著高于梗塞组（F=78.92, F=209.14,P<0.01），两组之间差异不明显（q=2.86,2.47,P>0.05）。结论尿激酶在脑梗死超早期有显著溶栓作用，低频超声波具有显著的协助尿激酶溶栓作用，可以减少尿激酶用量，MMP9可能在脑梗塞后溶栓的出血转化中起着重要作用。

关键词: 脑梗塞；超声检查；溶栓；大鼠，Wistar

Abstract:

To observe the effect of transcranial ultrasound enhanced thrombolysis by urokinase on expression of matrix metal loproteinase9(MMP9)during cerebral Infarction in rats and to study the role of MMP9 in thrombolysis. MethodsAnimal models of acute cerebral infarction were made by injecting autothrombsis into the left middle cerebral artery of male Wistar rats. Intravenous urokinase and transcranial ultrasound were given in 3 hours. Function of the nervous system was measured by NSS. The infarct volume was measured after TTC staining. MMP9 changes were observed by immunohistochemical staining. ResultsThe NSS score of the large dose urokinase group and the ultrasound plus small dose urokinase group at 24 hours after treatment were lower than that before treatment (t=12.59, t=15.31, P<0.01). The infarct volume of the urokinase group and the ultrasound plus small dose urokinase group were lower than that of the infarct group（F=125.99, q=18.02， q=20.62， P<0.01）,but there were no differences between them(q=2.59,P>0.05). There were no positive cells of MMP9 in the shamoperated group. The positive rate and optical density value of MMP9 in the urokinase group or the ultrasound plus urokinase group were more than those in the infract group（F=78.92, F=209.14,P<0.01）.There were no significant differences between them（q=2.86, and 2.47, P>0.05）.ConclusionTranscranial low frequency ultrasound enhanced thrombolysis by urokinase can accelerate the recovery of the nervous symptoms in acute cerebral infarction rats, diminishes the infarct volume and reduces the dosage of urokinase. MMP9 participates in thrombolysis of cerebral ischemia and plays an important role in

中图分类号:

R743.32

宋玉强1，2，毕建忠1. 低频超声辅助尿激酶溶栓对大鼠脑梗死基质金属蛋白酶9的影响[J]. 山东大学学报(医学版), 2009, 47(12): 50-53.

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