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山东大学学报(医学版) ›› 2009, Vol. 47 ›› Issue (12): 46-49.

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JNK1/2通路蛋白及凋亡基因Caspase3在高温致神经管畸形作用中的表达

刘雯1,马金龙1,高彦丽1,林万润1,郭成浩2   

  1. 1. 山东大学附属省立医院病理科, 济南 250021; 2. 山东大学医学院病理科, 济南 250012
  • 收稿日期:2009-09-17 出版日期:2009-12-16 发布日期:2009-12-16
  • 通讯作者: 马金龙(1963- )男,教授,硕士生导师,主要从事神经管畸形的研究。 Email:majinlong123@hotmail.com
  • 作者简介:刘雯(1983- ),女,硕士研究生,主要从事神经管畸形的研究。 Email:L-L5211@163.com
  • 基金资助:

    国家自然科学基金资助项目(30560045)。

Expressions of the JNK1/2 pathway and Caspase3 in the development of neural tube defects caused by Hyperthermia

LIU Wen 1 , MA Jinlong 1 , GAO Yanli 1 , LIN Wanrun 1 , GUO Chenghao 2   

  1. 1. Department of Pathology, Proviocial Hospital Affiliated to Shandong University, Jinan 250021, China;
    2. Department of Pathology, School of Medicine, Shandong University, Jinan 250012, China
  • Received:2009-09-17 Online:2009-12-16 Published:2009-12-16

摘要:

目的探讨高温致畸 MAPKs中JNK1/2通路蛋白及凋亡基因Caspase3的表达作用及其机理。方法孕鼠于受精第8天置42?℃水浴,持续20?min后,分别于2、8、16、24、48、72?h经麻醉处死剖腹取胎,并分为高温组(2~72?h,42?℃)和对照组(2~72?h,37?℃)。采用Western blot技术检测JNK1/2、PJNK1/2及Caspase3的表达量。结果高温组JNK1/2的表达量与对照组无明显差异,各组内不同时间的表达量无明显变化;PJNK1/2在对照组中不表达,高温组8~48?h时的表达量均高于相应的对照组(P<0.05),于16?h时表达增加最为显著;Caspase3在对照组中无表达,在高温组8~48?h时表达增加(P<0.05),于8?h时增加最为显著。结论高温刺激能促使JNK1/2发生磷酸化和凋亡基因Caspase3的活化,这可能是高温致胚胎先天性畸形的原因之一。

关键词: 高温,诱发;金仓鼠;畸形;有丝分裂素激活蛋白激酶激酶类;半胱氨酸天冬氨酸蛋白酶

Abstract:

To study expressions and  roles of the JNK1/2 pathway of MAPKs and Caspase3 in the development of neural tube defects caused by hyperthermia. MethodsPregnant golden hamsters in the hyperthermia group were immersed in hot water(42?℃) for 20?min on GD8. After treatment of 2, 8, 16, 24, 48 and 72?h, the hamsters were terminated after anesthesia and the embryos were removed from the fetal membrane, and divided into the hyperthermia group(272?h, 42?℃) and the control group(272?h, 37?℃). Expressions of the JNK1/2 pathway and PJNK1/2 and Caspase3 were measured by Western blot. ResultsExpression of JNK1/2 had no significant changes in the two groups. However, PJNK1/2 activity increased in the hyperthermia group at 848?h and the amount increased significantly compared with the control group(P<0.05), and the peak appeared at 16?h. Caspase3 activity had no significant changes in the control group but increased in the hyperthermia group, and the amount of Caspase3 increased compared with the control group at 848?h(P<0.05), and the peak appeared at 8?h. ConclusionThe PJNK1/2 pathway and Caspase3 were stimulated by hyperthermia, which maybe one of the pathway that induces the development of neural tube defects.

Key words: Hyperthermia, induced; Mesocricetus; Abnormalities; Mitogenactivated protein kinase kinases; Caspases

中图分类号: 

  • R321.6
[1] 李贵宝1,张卫民2,李鹏3. 内脏完全倒转1例[J]. 山东大学学报(医学版), 2012, 50(10): 132-132.
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