梗阻性肾病肾纤维化与血清中Ⅲ型胶原变化的相关性研究

山东大学学报(医学版) ›› 2009, Vol. 47 ›› Issue (12): 29-32.

梗阻性肾病肾纤维化与血清中Ⅲ型胶原变化的相关性研究

张亮¹，刘玉强¹，李远²，殷钢¹，孟凡伟³，柳豪⁴，王传运⁵

1. 山东大学齐鲁医院泌尿外科, 济南 250012； 2. 山东大学齐鲁医院 ICU，济南 250012；
3. 山东大学齐鲁医院生殖医学中心, 济南 250012； 4. 山东大学齐鲁医院急诊科, 济南 250012；
5. 济宁市第一人民医院泌尿外科，山东济宁 272100

收稿日期:2009-06-18 出版日期:2009-12-16 发布日期:2009-12-16
通讯作者: 刘玉强（1958- ），男，教授，主要从事泌尿外科疾病的研究。Email：lyq-sdql@163.com
作者简介:张亮（1982- ），男，硕士研究生，主要从事泌尿外科疾病的研究。

Relationship between collagen type Ⅲ in serum and renal fibrosis in obstructive nephropathy

ZHANG Liang ¹ , LIU Yuqiang ¹, LI Yuan ² ,　YIN Gang ¹, MENG Fanwei ³ , LIU Hao ⁴ , WANG Chuanyun ⁵

1. Department of Urology; 2. Department of ICU; 3. Department of Reproductive Medicine;
4. Department of Emergency, Qilu Hospital of Shandong University, Jinan 250012, China;
5. Department of Urology, Jining First People′s Hospital, Jining 272100, China

Received:2009-06-18 Online:2009-12-16 Published:2009-12-16

摘要/Abstract

摘要：

目的探讨梗阻性肾病时血清中Ⅲ型前胶原（PCⅢ）及Ⅲ型前胶原氨基末端肽（PⅢNP）浓度的变化与肾组织纤维化的相关性。方法将健康雄性新西兰大白兔40只通过手术分为单侧输尿管部分梗阻组、单侧输尿管完全梗阻组、假手术组、正常对照组，每组10只。术前及术后1～8周抽取大白兔静脉血，放射免疫法测定血清中PCⅢ及PⅢNP含量。术后第8周提取动物肾脏组织，免疫组化SP法染色测定Ⅲ型胶原含量，HE染色测定肾小管肾间质纤维化程度。结果①术后1～8周PCⅢ及PⅢNP值较术前升高（P＜0.05）。单侧输尿管部分梗阻组、单侧输尿管完全梗阻组PCⅢ及PⅢNP值较同期对照组、假手术组值升高（P＜0.05）;②单侧输尿管部分梗阻组、单侧输尿管完全梗阻组肾小管肾间质病变比率较对照组差异有统计学意义（P＜0.05）;③血清PCⅢ及PⅢNP浓度与病变肾小管肾间质比率呈正相关（P＜0.05）。结论血清PCⅢ及PⅢNP与肾积水时肾脏间质纤维化密切相关。在排除其他脏器纤维化的前提下，血清PCⅢ及PⅢNP可以作为早期判断肾积水肾脏纤维化的无创性检查指标。

关键词: 肾积水；肾纤维化；Ⅲ型胶原

Abstract:

To explore the relevance of collagen type Ⅲ in serum of obstructive nephropathy and renal fibrosis so as to elucidate the diagnosis value of serum PCⅢ and PⅢNP concentration for renal fibrosis. MethodsForty male New Zealand white rabbits were divided into four groups: the unilateral unilateral complete ureteral obstruction group, the unilateral partial ureteral obstruction group, the shamoperation group and the normal control group. The serum of each rabbit was reserved from the 1st to the 8th week before and after the operation. Collagen type Ⅲ was measured by immunohistochemical staining, and PCⅢ and PⅢNP were measured by radioimmunoassay（RIA）. The kidneys of all rabbits were taken out and stained by HE. Results①PCⅢ and PⅢNP in serum increased after the operation. ②Interstitial fibrosis was observed in the kidneys of rabbits of the unilateral complete ureteral and partial ureteral obstruction groups. ③PCⅢand PⅢNP in serum were positively correlated with the degree of glomerulosclerosis and tubuloinerstial injury. ConclusionPCⅢ and PⅢNP in serum are correlative with renal interstitial fibrosis in hydronephrosis. PCⅢ and PⅢNP in serum can be used to indicate renal fibrosis in hydronephrosis on the condition that other organ fibrosis should be excluded.

Key words: Hydronephrosis; Renal fibrosis; Collagen type Ⅲ

中图分类号:

R692.2

张亮1，刘玉强1，李远2，殷钢1，孟凡伟3，柳豪4，王传运5. 梗阻性肾病肾纤维化与血清中Ⅲ型胶原变化的相关性研究[J]. 山东大学学报(医学版), 2009, 47(12): 29-32.

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