葡萄糖耐量减低人群血小板相关功能研究

山东大学学报(医学版) ›› 2009, Vol. 47 ›› Issue (11): 9-13.

葡萄糖耐量减低人群血小板相关功能研究

刘燕¹，王红梅²,亓文波¹

1. 泰安市中心医院老年病三科，山东泰安 271000; 2. 肥城矿业中心医院老年病科，山东肥城 271608

收稿日期:2009-04-26 发布日期:2009-11-16
通讯作者: 亓文波（1956- ），女，主任医师，糖尿病及并发症的基础与临床。 Email:qwb125@sina.com
作者简介:刘燕（1971- ），男，主治医师，硕士，老年糖尿病的防治。
基金资助:
泰安市科技发展计划项目（200083016）

Platelet function in subjects with impaired glucose tolerance

LIU Yan ¹, WANG Hongmei ², QI Wenbo ¹

1. Department of Geriatrics, Taian Central Hospital, Taian 271000, Shandong, China;
2. Department of Geriatrics, Feicheng Mining Central Hospital, Feicheng 271608, Shandong, China

Received:2009-04-26 Published:2009-11-16

摘要/Abstract

摘要：

目的探讨糖耐量减低（IGT）人群血小板活化状态及影响因素。方法选择IGT组33例、糖尿病组和正常对照组各30例为研究对象。采用流式细胞术(FCM)检测血小板膜蛋白CD62P、血小板激活复合物(PAC1)阳性率和平均荧光强度(MFI)；电阻抗法测定血小板数目(count)、血小板平均体积(MPV)、血小板分布宽度(PDW)及大血小板比率(PLCR)等参数及总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDLC)、低密度脂蛋白胆固醇(LDLC)、极低密度脂蛋白胆固醇(VLDLC)、空腹血糖(FPG)和空腹胰岛素(FIns)。结果IGT组、糖尿病组CD62P%(P＜0.01)、CD62PMFI(P＜0.05)、PAC1%(P＜0.01)、MPV(P＜0.05)、PDW(P＜0.05)及PLCR%(P＜0.05)均较正常对照组升高；IGT组与糖尿病组之间差异无统计学意义(P＞0.05)。多元回归分析显示，IGT组与CD62P密切相关的因素依次为PLCR、LDLC、TC和体质量指数（BMI）；与PAC1相关的因素依次为PLCR、MPV及DBP。结论IGT人群存在与糖尿病同等程度的血小板活化；LDLC、TC、DBP及BMI是影响血小板活化的因素。

关键词: 糖耐量减低；CD62P；血小板激活复合物1；流式细胞术

Abstract:

To investigate the state of platelet activation and its influencing factors in subjects with impaired glucose tolerance(IGT). Methods33 patients with IGT were enrolled in this study. 30 DM and healthy persons served as the controls. Percentage and mean fluorescence intensity of CD62P, PAC1 as indexes of platelet activation were measured using whole blood flow cytometry (FCM). Changes of count of blood platelet, mean platelet volume(MPV), platelet distribution width (PDW) and plateletlarge cell ratio(PLCR)were analyzed with a blood cell counter. TC, TG, HDLC, LDLC, VLDLC, FPG and FIns were simultaneously measured. ResultsIGT and subjects with diabetes had higher levels of CD62P%(P＜0.01) and CD62MFI(P＜0.05) and PAC1% (P＜0.01) compared with the NC group. CD62P%,CD62MFI and PAC1% had no statistical differences between the IGT and DM groups(P＞0.05). By stepwise regression analysis, CD62P in the IGT group had relation to PLCR, LDLC, TC and body mass index (BMI) independently. PLCR, MPV and DBP were contributors to PAC1. ConclusionThere is the same degree of platelet activation in subjects with IGT, and DM. LDLC, TC, DBP and BMI are contributors to CD62P and PAC1 expressions on platelet in IGT.

Key words: Impaired glucose tolerance; CD62P; Platelet activated complex1; Flow cytometry

中图分类号:

R587

刘燕1，王红梅2,亓文波1. 葡萄糖耐量减低人群血小板相关功能研究[J]. 山东大学学报(医学版), 2009, 47(11): 9-13.

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