酒精性肝纤维化与DDR2关系及复方甘草酸苷治疗对其影响的实验研究

山东大学学报(医学版) ›› 2009, Vol. 47 ›› Issue (11): 80-84.

酒精性肝纤维化与DDR2关系及复方甘草酸苷治疗对其影响的实验研究

张玉泉¹，阎明¹，张喜红²，刘慧敏³

1. 山东大学齐鲁医院消化内科，济南 250012； 2. 聊城市人民医院急诊科，山东聊城 252000；
3. 烟台毓璜顶医院消化内科，山东烟台 264000

收稿日期:2009-03-30 发布日期:2009-11-16
通讯作者: 阎明（1956- ），男，博士，教授，博士生导师，主要从事酒精性肝病的基础与临床研究。 Email:ymylh@163.com
作者简介:张玉泉（1973- ），男，主治医师，硕士研究生，主要从事酒精性肝病的基础与临床研究。
基金资助:
山东省科技攻关计划项目（2007GG30002002）。

Relationship between DDR2 and alcoholic liver fibrosis and response of compound glycyrrhizin tablets on them: an experimental study

ZHANG Yuquan ¹, YAN Ming ¹ , ZHANG Xihong ² , LIU Huimin ³

1. Department of Digestive Diseases, Qilu Hospital of Shandong University, Jinan 250012, China;
2. Department of Emergency, Liaocheng People′s Hospital, Liaocheng 252000, Shandong, China;
3. Department of Digestive Diseases, Yantai Yuhuangding Hospital, Yantai 264000, Shandong, China

Received:2009-03-30 Published:2009-11-16

摘要/Abstract

摘要：

目的观察酒精性肝纤维化与盘状结构域受体2（DDR2）的关系以及复方甘草酸苷（GGT）治疗对二者的影响。方法48只大鼠随机分为正常对照组、模型组、停酒组、未停酒组、停酒治疗组及未停酒治疗组（n=8）。模型组酒精灌胃16周后，与正常对照组一起处死。其余4组于酒精灌胃16周后分别予以停酒、不停酒、停酒+GGT治疗、不停酒+GGT治疗4周,至20周末全部处死。检测各组血清透明质酸(HA)、层粘连蛋白(LN)、Ⅲ型前胶原(PCⅢ)及Ⅳ型胶原(CⅣ)含量，取肝组织进行HE染色及Masson染色观察其组织病理学变化，实时荧光定量PCR和Western blot蛋白印迹法分别检测肝组织DDR2 mRNA和蛋白表达。结果酒精灌胃16周后，大鼠肝组织呈明显肝纤维化病理表现，血清HA、LN、PCⅢ、CⅣ水平和肝组织胶原面密度及DDR2表达均较正常对照组升高(P＜0.01)。停酒治疗组与停酒未治疗组、未停酒治疗组与未停酒未治疗组比较，上述各指标均降低(P＜0.05)。结论CGT对酒精性肝纤维化具有一定的治疗作用，其作用可能与下调DDR2的表达相关。

关键词: 肝硬化，酒精性；复方甘草酸苷；盘状结构域受体2

Abstract:

To investigate the relationship between DDR2 and alcoholic liver fibrosis as well as the response to compound glycyrrhizin tablets treatment on them. Methods48 rats were randomly divided into 6 groups (8 in each group): the control group, the model group, the Alc disc group, the Alc contin group, the CGT + Alc disc group and the CGT + Alc contin group. After 16week intragastric administration with alcohol, rats in the model group were sacrificed, at the same time rats in the control group were killed. The rest having been treated by intrastric alcoholic for 16 weeks in the Alc disc group (stopped alcohol intragastric), the CGT + Alc disc group (stopped alcohol intragastric and treated by CGT), the Alc contin group (continued alcohol intragastric without treatment of CGT) and the CGT + Alc contin group (continued alcohol intragastric with treatment of CGT simultaneously) were sacrificed at the end of the 20th week. Levels of serum hyaluronic acid (HA), laminin (LN), procollagen type Ⅲ (PCⅢ) and collagen type Ⅳ (CⅣ) were determined. Pathologic changes of the liver were observed by HE staining and Masson staining. Expressions of DDR2 mRNA and protein were determined by RTPCR and Western blot, respectively. ResultsPathological liver fibrosis was obviously found in rats with alcoholic intragastric administration for 16 weeks. Areadensity percentage of collagen fibrosis, levels of serum HA, LN, PCⅢ and CⅣ, and levels of DDR2 mRNA and protein in the liver were significantly increased in these indexes were decreased in the CGT + Alc disc group compared with the Alc disc group and in the CGT + Alc contin group compared with the Alc contin group(P＜0.05). ConclusionCGT had certain therapeutic effects on alcoholic liver diseases, which may relate to downregulation of DDR2 expression.

Key words: Liver cirrhosis, alcoholic; Compound glycyrrhizin tablet; Discoidin domain receptor 2

中图分类号:

R975.5

张玉泉1，阎明1，张喜红2，刘慧敏3. 酒精性肝纤维化与DDR2关系及复方甘草酸苷治疗对其影响的实验研究[J]. 山东大学学报(医学版), 2009, 47(11): 80-84.

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