罗格列酮对肝星状细胞MMP2、TIMP1mRNA表达的影响

山东大学学报(医学版) ›› 2009, Vol. 47 ›› Issue (11): 74-75.

罗格列酮对肝星状细胞MMP2、TIMP1mRNA表达的影响

李森林，赵景润，任晓燕，马青竹，谢佳平，李振方，郎翠翠

聊城市人民医院消化内科，山东聊城 252000

收稿日期:2009-09-15 发布日期:2009-11-16
作者简介:李森林（1964- ）男，主任医师，硕士生导师，主要从事肝纤维化发病机制研究。 Email:lisenlin6201@163.com

Effects of the Rosiglitazone on expressions of MMP2 and TIMP1 mRNA in hepatic stellate cells

LI Senlin, ZHAO Jingrun, REN Xiaoyan, MA Qingzhu, XIE Jiaping, LI Zhenfang, LANG Cuicui

Department of Digestive Disease, Liaocheng People′s Hospital, Liaocheng 252000, Shandong, China

Received:2009-09-15 Published:2009-11-16

摘要/Abstract

摘要：

目的探讨罗格列酮对体外培养肝星状细胞MMP2及TIMP1基因表达的影响。方法采用HSCT6肝星状细胞系，经不同浓度的罗格列酮干预，采用RTPCRA法检测各组MMP2及TIMP1基因的表达情况。结果在10、20?μmol/L罗格列酮组，TIMP1的表达较空白对照组明显降低(P＜0.05)，而MMP2的表达与空白对照组相比差异无统计学意义。结论罗格列酮的抗肝纤维化作用与抑制HSC表达TIMP1有关。

关键词: 罗格列酮；肝星状细胞；基质金属蛋白酶2；金属蛋白酶组织抑制因子1

Abstract:

To investigate the effects of Rosiglitazone on expressions of MMP2 and TIMP1 mRNA in hepatic stellate cells (HSCs) in vitro. MethodsThe HSC line, HSCT6 was incubated with different concentrations of Rosiglitazone. The MMP2 and TIMP1 mRNA levels were measured by reverse transcription polymerase chain reaction (RTPCR). ResultsExpression of TIMP1 mRNA in the Rosiglitazonetreatment groups was significantly lower than that in the control group (P＜0.05), and there was no difference in the MMP2 mRNA levels between the Rosiglitazonetreatment groups and the control group. ConclusionThe antifibrosis mechanism of Rosiglitazone is partly due to its downregulation on TIMP1 but not on MMP2 expression of hepatic stellate cells.

Key words: Rosiglitazone; Hepatic stellate cells; Matrix metalloproteinases2; Tissue inhibitor of metalloproteinases

中图分类号:

R575

李森林，赵景润，任晓燕，马青竹，谢佳平，李振方，郎翠翠. 罗格列酮对肝星状细胞MMP2、TIMP1mRNA表达的影响[J]. 山东大学学报(医学版), 2009, 47(11): 74-75.

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