Dishevelled2和Vangle2蛋白在过量维甲酸致昆明小鼠腭裂发生中的表达变化及意义

山东大学学报(医学版) ›› 2009, Vol. 47 ›› Issue (11): 29-33.

Dishevelled2和Vangle2蛋白在过量维甲酸致昆明小鼠腭裂发生中的表达变化及意义

尹海燕¹，卓煜娅¹，邹维艳¹，孙美群¹，耿慧慧³，刘凯²

1. 蚌埠医学院组织学与胚胎学教研室，安徽蚌埠 233030；
2. 山东大学医学院组织学与胚胎学教研室，济南 250012;
3. 蚌埠医学院人体解剖学教研室，安徽蚌埠 233030

收稿日期:2008-12-18 发布日期:2009-11-16
通讯作者: 刘凯（1947- ），男，教授，主要从事实验胚胎学研究。Email:liukai@sdu.edu.cn
作者简介:尹海燕（1981- ），女，硕士,助教，主要从事实验胚胎学研究。
基金资助:
山东省自然科学基金资助课题（Y2005C63)。

Effects of Dishevelled2 and Vangl2 on the development of cleft palate induced by excess retinoid acid in KM mice

YIN Haiyan, ZHUO Yuya, ZOU Weiyan, SUNMeiqun, GENG Huihui, LIU Kai

1. Department of Histology and Embryology, Bengbu Medical College, Bengbu 233030, Anhui, China;
2. Department of Histology and Embryology, Sciool of Medicine,Shandong University, Jinan 250012, China;
3. Department of Human Anatomy, Bengbu Medical College, Bengbu 233030, Anhui, China

Received:2008-12-18 Published:2009-11-16

摘要/Abstract

摘要：

目的探讨Dishevelled2和Vangl2蛋白在过量维甲酸致昆明小鼠腭裂发生中的作用。方法采用免疫组织化学及图像分析方法,检测维甲酸致畸小鼠（实验组）和相应对照组小鼠腭板中Dishevelled2和Vangl2蛋白的表达状况。结果Dishevelled2在实验组间充质的表达量明显高于相应对照组，在腭板上皮表达量先明显高于而后又明显低于相应对照组；Vangl2在实验组腭板间充质中的表达量先明显高于而后又明显低于相应对照组。结论Dishevelled2在间充质细胞中表达的上调以及在上皮细胞中先上调又明显下调和Vangl2在腭板间充质细胞中表达的先上调后下调可能与维甲酸致昆明小鼠腭裂发生相关。

关键词: 维甲酸；腭裂；Dishevelled2蛋白;Vangl2蛋白;免疫组织化学

Abstract:

To explore the effects of Dishevelled2 and Vangl2 protein on cleft palate induced by excess retinoid acid in KM mice. MethodsIn both malformed embryos induced by excess retinoid acid (the experimental group) and normal KM mice (the control group), expressions of Dishevelled2 and Vangl2 in the epithelium of the palatal shelves and the mesenchyma were determined by immunohistochemistry and image analysis. ResultsExpression of Dishevelled2 in the mesenchyma of the experimental group was significantly higher than that in the control group, however that in the epithelium of the palatal shelves of the experimental group was significantly higher, but then lower than that in the control group. So was expression of Vanlg2 in the mesenchyma. ConclusionExcess retinoid acid may interfere with normal expression of Dishevelled2 and Vangle protein in developing palates and causes cleft palate in KM mice.

Key words: Retinoic acid; Cleft palate; Dishevelled2; Vangl2; Immunohistochemistry

中图分类号:

R321.6

尹海燕1，卓煜娅1，邹维艳1，孙美群1，耿慧慧3，刘凯2. Dishevelled2和Vangle2蛋白在过量维甲酸致昆明小鼠腭裂发生中的表达变化及意义[J]. 山东大学学报(医学版), 2009, 47(11): 29-33.

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