腮腺癌在多形性腺瘤中p16基因的表达及甲基化改变

山东大学学报(医学版) ›› 2009, Vol. 47 ›› Issue (11): 108-110.

腮腺癌在多形性腺瘤中p16基因的表达及甲基化改变

李海燕¹，郭小玲²，王建华²，曲伟栋²，赵华强²

1. 济南烟草职工医院，济南 250100; 2. 山东大学口腔医院, 济南 250012

收稿日期:2008-10-11 发布日期:2009-11-16
通讯作者: 赵华强(1964- ),男, 博士,副教授,主要从事口腔颌面外科、颌面部整形研究。 Email:zhaohq19@163.com
作者简介:李海燕(1971- ),女,主治医师,主要从事口腔肿瘤研究。
基金资助:
山东省医药卫生科技发展计划资助项目(2005HW043)。

Expression and methylation of p16 in carcinoma ex pleomorphic adenoma of the parotid gland

LI Haiyan, GUO Xiaoling, WANG Jianhua, QU Weidong, ZHAO Huaqiang

1. Workers Hospital of Jinan Tobacco Group, Jinan 250100, China;
2. Department of Oral Maxillofacial Surgery, Hospital of Stomatology， Shangdong University, Jinan 250012, China

Received:2008-10-11 Published:2009-11-16

摘要/Abstract

摘要：

：目的研究腮腺癌在多形性腺瘤中（carcinoma ex pleomorphic adenoma，CXPA）p16基因的蛋白表达和甲基化改变，并评价其在该肿瘤癌变中的意义。方法收集40例腮腺CXPA标本及10例正常腮腺标本，应用免疫组织化学技术和甲基化特异性PCR技术，检测p16基因的蛋白表达和甲基化改变。结果正常腺体p16蛋白表达均为阳性，21例 (21/40，52.5%)CXPA标本表现为表达降低或表达缺失，二者存在统计学差异(P＜0.05)。正常腺体p16基因启动子未见高甲基化，16例(16/40, 40%)CXPA出现启动子高甲基化，二者存在统计学差异(P＜0.05)。结论p16基因表达降低或表达缺失、启动子高甲基化对于腮腺CXPA的癌变具有重要意义，后者可能是p16基因在CXPA中的重要失活机制。

关键词: 癌在多形性腺瘤中；基因；免疫组织化学；DNA甲基化

Abstract:

To study the changes of p16 gene protein expression and promoter methylation in carcinoma ex pleomorphic adenoma (CXPA) of the parotid gland, and to evaluate their significance for carcinogenesis of CXPA. Methodsp16 gene protein expression and promoter methylation were determined in 40 samples of CXPAs of the parotid glands and 10 normal samples of the parotid gland(NM) using immunohistochemistry (IHC) and methylation specific PCR (MSP). ResultsIn normal parotid glands, the p16 protein was positively expressed. In the CXPAs group, 21 cases (52.5%) showed a decreased (in different degrees) or absence of p16 protein expression. There was significant statistical difference (P＜0.05) between the two groups. Hypermethylation of the p16 gene promoter was found in 16 cases (16/40, 40%) of CXPAs, but was not found in normal salivary glands (P＜0.05). ConclusionThe decreased or absence of p16 protein expression and promoter hypermethylation play an important role in the carcinogenesis of CXPA. And hypermethylation may be the important inactivation mechanism of the p16 gene in CXPA.

Key words: Carcinoma ex pleomorphic adenoma; Gene; Immunohistochemistry; DNA methylation

中图分类号:

R739.87

李海燕1，郭小玲2，王建华2，曲伟栋2，赵华强2. 腮腺癌在多形性腺瘤中p16基因的表达及甲基化改变[J]. 山东大学学报(医学版), 2009, 47(11): 108-110.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed