内毒素脂多糖诱导人牙髓细胞凋亡的机制

山东大学学报(医学版) ›› 2009, Vol. 47 ›› Issue (11): 100-102.

内毒素脂多糖诱导人牙髓细胞凋亡的机制

张彦聪，张志芹

德州学院医学系，山东德州 253000

收稿日期:2009-03-20 发布日期:2009-11-16
作者简介:张彦聪（1980- ），女，硕士，助教，主要从事口腔细胞学基础研究。

Effects of Lipopolysaccharide on the proliferation and apoptosis of human dental pulp cells

ZHANG Yancong， ZHANG Zhiqin

DezhouUniversity Department of Medicine, Dezhou 253000， Shandong， China

Received:2009-03-20 Published:2009-11-16

摘要/Abstract

摘要：

目的观察内毒素脂多糖(lipopolysaccharide, LPS)对体外培养的人牙髓细胞的致凋亡作用及其对凋亡相关的半胱氨酸蛋白酶3(caspase3)、B细胞淋巴瘤2（Bcl2）、B细胞淋巴瘤相关蛋白X（Bax）活性的影响。方法用不同浓度(0、0.01、0.10、1.00、10.00、100.00?mg/L)的LPS作用于细胞,采用MTT染色法检测LPS对细胞增殖的影响；采用流式细胞术检测细胞周期及凋亡；采用免疫组织化学染色技术检测细胞的caspase3、Bcl2、Bax表达变化。结果不同浓度LPS组细胞的生长与对照组相比均受到显著的抑制(P＜0.05),细胞增殖抑制率呈剂量依赖关系；流式细胞术检测结果显示,不同浓度的LPS作用细胞72?h,细胞的凋亡率分别为3.1%、3.5%、12.1%、14.4%、24.3%、59.7%；免疫组织化学染色显示Bax表达增强，Bcl2、caspase3表达减弱。结论LPS可显著地抑制人牙髓细胞增殖，诱导细胞凋亡，降低Bcl2、caspase3活性，升高Bax活性,并呈明显的量效关系。

关键词: 牙髓；内毒素；细胞凋亡；半胱氨酸蛋白酶3；B细胞淋巴瘤2

Abstract:

To observe lipopolysaccharide(LPS) induced apoptosis of human dental pulp cells(HDPC) and its effect on apoptosisrelated cystine protease 3 (caspase3), Bcl2, and Bax activity in vitro. MethodsPulp cells were stimulated with 0, 0.01, 0.10, 1.00, 10.00, 100.00?mg/L LPS. Then the cell proliferation activity was detected using MTT colorimetrics, the cell cycle and apoptosis after LPS stimulation using flow cytometry, and expressions of caspase3, Bcl2, Bax using immunocytochemical staining. ResultsCell growth was significantly inhibited by LPS in a dosedependent manner. Flow cytometry results showed that the apoptotic rates were respectively 3.1%, 3.5%, 12.1%, 14.4%, 24.3%, and 59.7% after stimulated by LPS. Immunocytochemical staining showed that Bax expression was increased, while caspase3 and Bcl2 expressions were decreased. ConclusionLPS could significantly inhibit the proliferation of HDPC, induces apoptosis, reduces Bcl2 and caspase3 activity, and increases Bax activity, which is dosedependent.

Key words: Dental pulp; Lipopolysaccharide; Apoptosis; Caspase3; Bcl2

中图分类号:

R781.2

张彦聪，张志芹. 内毒素脂多糖诱导人牙髓细胞凋亡的机制[J]. 山东大学学报(医学版), 2009, 47(11): 100-102.

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