子宫腺肌病中PTEN和Akt的表达及其相关性研究

山东大学学报(医学版) ›› 2009, Vol. 47 ›› Issue (10): 86-90.

子宫腺肌病中PTEN和Akt的表达及其相关性研究

刘姝，张辉，赵兴波，李继俊，李明江

山东大学附属省立医院妇产科，济南 250021

收稿日期:2009-02-25 出版日期:2009-10-16 发布日期:2009-10-16
通讯作者: 李明江（1963- ），男，博士，副主任医师，主要从事妇科肿瘤和妇科内分泌研究。Email： limingjiang1963@126.com
作者简介:刘姝（1980- ），女，硕士研究生，主要从事妇科肿瘤和妇科内分泌研究。Email：liu-shu-good@126.com
基金资助:
国家自然科学基金资助课题（30571960）；山东省自然科

学基金资助课题(2004ZX04)。

Expressions of PTEN and Akt and relationship to adenomyosis

LIU Shu, ZHANG Hui, ZHAO Xingbo, LI Jijun, LI Mingjiang

Department of Obstetrics and Gynecology, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China

Received:2009-02-25 Online:2009-10-16 Published:2009-10-16

摘要/Abstract

摘要：

目的探讨子宫腺肌病病灶中10号染色体缺失张力蛋白磷酸酶(PTEN)和蛋白激酶B（PKB/Akt）的蛋白表达情况，旨在为子宫腺肌病的发生发展以及治疗提供理论依据。方法采用免疫组织化学SP法检测子宫腺肌病异位内膜(30例)、在位内膜（27例）、正常子宫内膜(20例)的腺上皮细胞和间质细胞中PTEN、Akt、磷酸化Akt （PAkt）蛋白的表达并进行比较。结果PTEN蛋白在腺上皮细胞中的表达，正常子宫内膜表达高于在位内膜和异位内膜（P＜0.05，P＜0.01），子宫腺肌病在位内膜的表达高于子宫腺肌病异位内膜的表达（P＜0.01)；Akt、PAkt蛋白在子宫腺肌病异位内膜的腺上皮细胞中的表达强度高于在位内膜（P＜0.05）和正常子宫内膜(P＜0.01),其在位内膜腺上皮细胞中的表达强度高于正常子宫内膜(P＜0.01)。PTEN蛋白在间质细胞中的表达，正常子宫内膜明显高于子宫腺肌病的在位内膜和异位内膜（P＜0.05），但在位内膜与异位内膜之间比较差异无统计学意义（P＞0.05）；Akt、PAkt蛋白在间质细胞中的表达，异位和在位内膜明显高于正常内膜，异位内膜和在位内膜两组之间比较差异无统计学意义（P＞0.05）。PTEN与Akt、PTEN与PAkt在子宫腺肌病异位内膜、在位子宫内膜和正常子弓内膜中的表达均呈负相关（r＝-0.444；r＝-0.546）。结论PTEN蛋白表达在子宫腺肌病的在位、异位内膜中减少；Akt、PAkt蛋白表达增加。PTEN蛋白低表达不能有效的抑制Akt的激活，从而对子宫腺肌病的发生与发展起重要的作用。

关键词: PTEN蛋白, Akt蛋白, PAkt蛋白, 子宫腺肌病, 子宫内膜异位症

Abstract:

To explore expressions of PTEN and Akt proteins in adenomyosis tissues, and to provide a theoretic basis on the development, progress and therapy of adenomysis. MethodsImmunohistochemical staining with SP method was used to determine expression of PTEN, Akt and PAkt in the ectopic lesions with myometrium of adenomyosis group(30 cases), the eutopic endometrium group (27 cases), and the normal endometrium group (20 cases). ResultsIn gland cells, PTEN protein expression in the ectopic lesion group was the lowest among the three groups. The PTEN expression in the normal endometrium group was the highest in them（P＜0.01). Expressions of Akt and PAkt proteins in the ectopic lesion of adenomyosis group were higher than those in the eutopic endometrium in adenomyosis group（P＜0.05) and significantly higher than those in the normal endometrium (P＜0.01). Expressions of Akt and PAkt in eutopic endometrium with adenomyosis were higher than those of normal endometrium (P＜0.01). In stroma cells, the PTEN protein expression in ectopic and eutopic lesions of adenomyosis was significantly lower than that in normal endometrium, but there was no statistical significance between the two groups. Expressions of Akt and PAkt proteins in ectopic and eutopic lesions of adenomyosis were significantly higher than those in normal endometrium， but also there were no statistical differences. There were negative correlations among expressions of Akt, PAkt, and PTEN in ectopic lesions , eutopic endometrium of adenomyosis and normal endometrium（r＝-0.444,r＝-0.546）. ConclusionWhile PTEN protein expression is low, expressions of Akt and PAkt proteins are high inectopic and eutopic lesions of 〖KG*3]adenomyosis.Hypoexpression of PTEN can not inhibit abnormal activation of Akt, which might play an important role in the pathogenesis and development of adenomyosis.

Key words: PTEN protein; Akt protein; PAkt protein; Adenomyosis; Endometriosis

中图分类号:

R711.71

. 子宫腺肌病中PTEN和Akt的表达及其相关性研究 [J]. 山东大学学报(医学版), 2009, 47(10): 86-90.

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