羊膜匀浆液对兔小梁切除术后滤过泡及TGFβ1、CTGF表达的影响

山东大学学报(医学版)

羊膜匀浆液对兔小梁切除术后滤过泡及TGFβ1、CTGF表达的影响

高髻云¹, 张伟²

山东大学 1 医学院，济南 250012； 2. 附属千佛山医院眼科，济南 250014

收稿日期:2007-07-06 修回日期:1900-01-01 出版日期:2008-02-16 发布日期:2008-02-16
通讯作者: 张伟

Effects of human amniotic homogenate on blebs and expressions of TGFβ1 and CTGF after experimental trabeculectomies in rabbits

GAO Ji-yun¹, ZHANG Wei²

1. School of Medicine, Shandong University, Jinan 250012, China; 2. Department of Ophthalmology, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, China

Received:2007-07-06 Revised:1900-01-01 Online:2008-02-16 Published:2008-02-16
Contact: ZHANG Wei

摘要/Abstract

摘要： 目的研究羊膜匀浆提取液在兔实验性小梁切除术后对滤过泡及TGFβ1、CTGF表达的影响。方法将30只新西兰大白兔随机平均分为实验组和对照组,选右眼行标准小梁切除术。实验组于术后当天开始每天用羊膜匀浆液点眼，对照组单纯用氯霉素滴眼液点眼。在不同时间点分别作临床及病理观察。结果共22眼形成功能性滤过泡，其中实验组14眼，对照组8眼(P＜0.05)。眼压除术前与术后2d差异无统计学意义(P＞0.05)外，其余观察点差异均有统计学意义(P＜0.05)。实验组成纤维细胞(Fb)数目较对照组少(P＜0.05)。免疫组化染色观察TGFβ1、CTGF的表达，实验组低于对照组（P＜0.05）。结论羊膜匀浆上清液点眼无并发症发生，且能增强滤过泡滤过功能及降眼压效果，对滤过泡TGFβ1、CTGF表达有抑制作用。

Abstract: To investigate the effects of human amniotic homogenate on blebs and the expressions of TGFβ1 and CTGF following trabeculectomy in rabbits. MethodsThirty New Zealand white rabbits were randomly divided into two groups: the control group and the experimental group. The right eyes were subjected to standard trabeculectomies. After the operations, human amniotic homogenate was topically used in the experimental group four times daily for 14 days and was not used in the control group. The clinical features and the pathological changes were observed on different days after surgeries. Results14 eyes in the experimental group had functional blebs and 8 in the control group(P＜0.05). The intraocular pressure of the control group was higher than that of the experimental group after 2 days（P＜0.05). The fibroblasts in the experimental group were less than those in the control group(P＜0.05). The expressions of TGFβ1 and CTGF were lower in the experimental group than those in the control group(P＜0.05). Conclusions The supernatant of a homogenized amnion has no side effects, and it can inhibit the expressions of TGFβ1 and CTGF and improves the filtering function and decreases the intraocular pressure.

Key words: Human amniotic homogenate, TGFβ1, CTGF, Glaucoma, Trabeculectomy

中图分类号:

R772

高髻云,张伟 . 羊膜匀浆液对兔小梁切除术后滤过泡及TGFβ1、CTGF表达的影响[J]. 山东大学学报(医学版), 2008, 46(2): 163-166.

GAO Ji-yun, ZHANG Wei. Effects of human amniotic homogenate on blebs and expressions of TGFβ1 and CTGF after experimental trabeculectomies in rabbits[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(2): 163-166.

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