苯丁酸钠体外诱导脑胶质瘤
细胞分化的实验研究

山东大学学报(医学版) ›› 2008, Vol. 46 ›› Issue (12): 1162-1165.

苯丁酸钠体外诱导脑胶质瘤
细胞分化的实验研究

李科，王志云，王波，张源，展如才，王成伟

1. 山东大学医学院，济南 250012； 2. 潍坊市人民医院，山东潍坊 261042；3. 济南市第三人民医院神经外科，济南 250101； 4. 山东大学第二医院神经外科，济南 250033

收稿日期:2008-04-08 出版日期:2008-12-16 发布日期:2009-12-16
作者简介:李科（1984- ），男，硕士研究生，主要从事脑胶质瘤的基础与临床研究。
基金资助:
山东省优秀中青年科学家科研奖励基金（02BS079）；1020人才工程资助课题。

Sodium phenylbutyrate (SPB) induces differentiation of the C6 glioma cells in vitro: an experimental study

LI Ke, WANG Zhiyun, WANG Bo, ZHANG Yuan, ZHAN Rucai, WANG Chengwei

1. School of Medicine, Shandong University, Jinan 250012, China; 2. Weifang People′s Hospital, Weifang 261042, Shandong, China; 3. Department of Neurosurgery, Jinan Third People′s Hospital, Jinan 250101, China; 4. Department of Neurosurgery, Second Hospital of Shandong University, Jinan 250033, China

Received:2008-04-08 Online:2008-12-16 Published:2009-12-16
Contact: 王成伟（1968- ），男，医学博士，主任医师，主要从事脑胶质瘤的基础与临床研究。

摘要/Abstract

摘要：

目的探讨苯丁酸钠（SPB）对脑胶质瘤C6细胞的增殖抑制、诱导凋亡作用。方法 C6细胞分别经1、2、4mmol/L SPB处理后，应用光镜观察细胞形态学变化；电镜观察细胞超微结构变化；MTT法检测细胞增殖情况；流式细胞仪检测细胞周期；软琼脂克隆形成率检测法测定克隆形成率；免疫组织化学染色检测GFAP表达。结果 C6胶质瘤细胞的光镜下、电镜下的结构改变显示细胞分化趋于成熟；MTT显示胶质瘤细胞增殖受到抑制；各浓度SPB处理组细胞处于G0/G1期细胞数增加(P＜0.01)，而处于S期细胞数减少(P＜0.01)，4mmol/L SPB处理组可见凋亡峰；软琼脂克隆形成率检测示克隆形成率降低(P＜0.01)；GFAP表达增多。结论苯丁酸钠可明显抑制C6细胞增殖，诱导细胞分化，甚至诱发细胞凋亡。

关键词: 苯丁酸钠；脑肿瘤；细胞分化；细胞凋亡

Abstract:

Objective To investigate the effects of sodium phenylbutyrate (SPB) on proliferation inhibition and apoptosis induction of the brain glioma cells. Methods The C6 glioma cells were first induced by 1, 2, 4mmol/L SPB, then the morphological changes of the C6 cells were observed by a light microscope; the ultra-structure of the cells were observed by an electron microscope; cell proliferation was determined by MTT assay; cell cycle was analyzed by flow cytometry; cell colony formation rate was measured by the soft agar clone forming test; and expression of GFAP was determined by immunohistochemistry staining. Results The morphology and ultrastructure of the C6 cells were differentiated towards normal cells; cell proliferation was inhibited by MTT assay; cells that stayed at the G0/G1 phage were increased (P＜0.01), while those that stayed at the S phage were decreased (P＜0.01), and cell apoptosis peaked in the 4mmol/L SPB group as shown by flow cytometry; cell cloning efficiency was degraded (P＜0.01) by the soft agar clone forming test; and expression of GFAP was increased. Conclusions SPB can apparently inhibit the proliferation of C6 cells and can induce cell differentiation and even apoptosis of C6 cells.

Key words: Sodium phenylbutyrate; Brain neoplasms; Cell differentiation; Apoptosis

中图分类号:

R739.41

李科，王志云，王波，张源，展如才，王成伟. 苯丁酸钠体外诱导脑胶质瘤
细胞分化的实验研究[J]. 山东大学学报(医学版), 2008, 46(12): 1162-1165.

LI Ke, WANG Zhiyun, WANG Bo, ZHANG Yuan, ZHAN Rucai, WANG Chengwei. Sodium phenylbutyrate (SPB) induces differentiation of the C6 glioma cells in vitro: an experimental study[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(12): 1162-1165.

参考文献

相关文章 8

[1]	牛俊婕1,王晗1,2,徐英1，周勇1，姜玉华1. 丙戊酸钠增强大鼠胶质瘤C6细胞放射敏感性的体外实验[J]. 山东大学学报(医学版), 2013, 51(06): 15-19.
[2]	王通，刘玉光，张良文，王宏伟，李连陵,刘志国. 阻断EGFR和mTOR信号通路对C6胶质瘤及其干细胞的影响[J]. 山东大学学报(医学版), 2013, 51(5): 37-43.
[3]	姚军1,2，齐宏旭3，范晓勇4，倪石磊1,2，王建刚1,2，李新刚1，2. 替莫唑胺缓释系统的构建及体外抗胶质瘤细胞的实验研究[J]. 山东大学学报(医学版), 2012, 50(9): 6-10.
[4]	郑鲲鹏1，宋辉2，樊明德1，张源3，李科1，王成伟1. h17细胞相关因子在人脑胶质瘤中的测定[J]. 山东大学学报(医学版), 2012, 50(6): 87-91.
[5]	尹俊毅1，李刚1，刘青林1，袁光1，张建1，曲迅2. 去甲肾上腺素对胶质母细胞瘤侵袭迁移能力的影响及机制研究[J]. 山东大学学报(医学版), 2012, 50(2): 16-20.
[6]	王晗1，李书鹏2，姜玉华1，刘芳2 . 谷胱甘肽对多巴胺诱导的GH4细胞凋亡的保护作用[J]. 山东大学学报(医学版), 2011, 49(11): 48-.
[7]	吴敏1，李刚2，孙金龙3，江玉泉2，王洁贞4. 颅内肿瘤危险因素的病例对照研究[J]. 山东大学学报(医学版), 2010, 48(4): 147-150.
[8]	王海萍李增伟迟兆富. 大鼠脑缺血再灌注损伤后脑组织MMP-9的表达及神经调节素的干预作用[J]. 山东大学学报(医学版), 2009, 47(5): 78-82.

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed