卡莫氟对强直性脊柱炎Th1/Th2类细胞因子表达影响的研究

山东大学学报(医学版)

卡莫氟对强直性脊柱炎Th1/Th2类细胞因子表达影响的研究

杨清锐¹，张源潮¹，李　鸣¹，孙红胜¹，牟　坤²

山东大学 1.山东省立医院风湿免疫科，山东济南 250021；2.医学院病理学教研室，山东济南 250012

收稿日期:2006-09-01 修回日期:1900-01-01 出版日期:2007-01-24 发布日期:2007-01-24
通讯作者: 杨清锐

Role of 1-Hexylcarbamoyl-5-Fluorouracil on the Th1/Th2 balance in patients with ankylosing spondylitis

YANG Qing-rui¹，ZHANG Yuan-chao¹，LI Ming¹，SUN Hong-sheng¹，MU Kun²

1. Department of Rheumatology and Immunology, Shandong Provincial Hospital,Shandong University, Jinan 250021, Shandong, China；2. Department of Pathology, School of Medicine, Shandong University, Jinan 250012, Shandong, China

Received:2006-09-01 Revised:1900-01-01 Online:2007-01-24 Published:2007-01-24
Contact: YANG Qing-rui

摘要/Abstract

摘要： 目的：观察卡莫氟(1-hexylcarbamoyl-5-fluorouracil,HCFU) 和柳氮磺胺吡啶(sulfalazine,SSZ)治疗强直性脊柱炎(ankylosing spondylitis, AS)后体内Th1/Th2类细胞因子的变化。方法：19例AS患者随机分为2组，分别给予HCFU(9例)和SSZ （10例），RT-PCR检测其治疗前后外周血单个核细胞(peripheral blood mononuclear cells,PBMC)中细胞因子γ-干扰素（IFN-γ）、白细胞介素-2（IL-2）、肿瘤坏死因子-α（TNF-α）、白细胞介素-4（IL-4）、白细胞介素-6（IL-6）mRNA片段的表达。结果:HCFU治疗后AS患者PBMC中IFN-γ、TNF-α mRNA的表达率分别为33.33%、44.44%，与治疗前相比差异有统计学意义（P＜0.05），IL-2、IL-4及IL-6 mRNA的表达差异无统计学意义。SSZ治疗后AS患者PBMC中IFN-γ mRNA的阳性表达率为70%,半定量结果显示，治疗后PBMC中IFN-γ mRNA的表达水平明显降低（P＜0.05)。80% AS患者在治疗后PBMC中IL4 mRNA呈阳性表达，较治疗前21.05%明显升高（P＜0.05）。IL-2、IL-6和TNF-α mRNA的表达无明显变化（P＞0.05）。结论：HCFU能使TNF-α mRNA表达降低，促进AS患者的免疫应答从Th1型向Th2型转变，小剂量即可使病情缓解。SSZ未发现对TNF-α mRNA表达有明显影响，可促使AS患者的免疫应答从Th1型向Th2型转变。

Abstract: Objective: To investigate the influence of sulfalazine (SSZ) and 1-Hexylcarbamoyl-5-Fluorouracil (HCFU) on T helper cytokines in patients with ankylosing spodylitis (AS). Methods: Nineteen AS patients were randomized treated with SSZ or HCFU. The RT-PCR semi-quantitative method was employed to determine the mRNA expression of Th1/Th2 cytokines (IFN-γ, IL-2, IL-4, IL-6 and TNF-α). The changes of Th1/Th2 subgroups modulated by SSZ and HCFU was studied. Results: HCFU administration was followed by a decrease expression of IFN-γ mRNA and TNF-α mRNA (33.33% vs 89.74%, 44.44% vs 94.74%, P＜0.05), whereas other cytokines (IL-2,IL-4, IL-6) produced no significant changes(P＞0.05). SSZ administration was followed by an increased expression of IL-4 mRNA ( 80% vs 21.05%, P＜0.05) and a decrease expression of IFN-γ mRNA (P＜0.05), whereas other cytokines (IL-2, IL-6, and TNF-α) produced no significant changes(P＞0.05). Conclusion: HCFU and SSZ therapy for AS patients results in a shift from Th1 to Th2 subsets. HCFU can decrease the mRNA levels of TNF-α.

Key words: Spondylitis,ankylosing, Th1 cell, Th2 cell, Sulfalazine, 1-Hexylcarbamoyl-5-Fluorouracil

中图分类号:

R593.23

杨清锐,张源潮,李　鸣,孙红胜,牟　坤 . 卡莫氟对强直性脊柱炎Th1/Th2类细胞因子表达影响的研究[J]. 山东大学学报(医学版), 2007, 45(1): 31-34.

YANG Qing-rui,ZHANG Yuan-chao,LI Ming,SUN Hong-sheng,MU Kun. Role of 1-Hexylcarbamoyl-5-Fluorouracil on the Th1/Th2 balance in patients with ankylosing spondylitis[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(1): 31-34.

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