酒精对慢性乙型肝炎患者乙肝病毒多位点基因突变影响研究

山东大学学报(医学版)

酒精对慢性乙型肝炎患者乙肝病毒多位点基因突变影响研究

许建民¹,陈建¹,王敏²,刘福国¹

山东大学齐鲁医院 1. 消化科； 2. 干部保健科, 山东济南 250012

收稿日期:2006-03-08 修回日期:1900-01-01 发布日期:2006-11-24
通讯作者: 许建民

XU JIan-min,CHEN Jian,WANG Min,LIU Fu-guo

Received:2006-03-08 Revised:1900-01-01 Published:2006-11-24
Contact: XU JIan-min

摘要/Abstract

摘要： 目的：探讨酒精对HBV DNA多位点基因变异的影响，为慢性乙型肝炎的临床诊断和治疗提供依据。方法:选取45例慢性乙肝患者为研究对象，分为嗜酒组和非嗜酒组，采用DNA芯片技术, 检测HBV DNA前C区1896及1814位点、BCP区1762及1764位点、P区528及552位点的基因变异。结果:嗜酒组在BCP区1762 、1764位点的变异率明显高于非嗜酒组(P均＜0.05)。在前C区1896、1814位点，P区528、552位点变异率差异无统计学意义（P均＞0.05）。结论:酒精可导致乙肝病毒BCP区1762 、1764位点发生基因突变，BCP区基因变异可促进HBV在人体内的复制表达，加重慢性乙型肝炎患者的病情。

关键词: 炝?丌坙?愌恢Ｇ耛炝疎毅?丌坙?耛炝?鄌籒悾Ｇ圛固叠忘＂圛固苠犯G

Abstract: To study the alcohol influence on gene mutations of HBV DNA and to offer testimony for clinical diagnosis and treatment of chronic hepatitis B. Methods: Twenty alcoholic patients with chronic hepatitis B and twentyfive nonalcoholic patients were enrolled in this study. Gene chip technique was used to detect gene mutations located in PreC nt1896 and nt1814, basal core promoter (BCP) nt1762 and nt1764, P nt528 and nt552. Results: The ratios of mutation on BCP nt1762 and nt1764 in alcoholic patients were significantly higher than nonalcoholic patients (P＜0.05). The ratios of mutation on Prec nt1896 nt1814 and P nt528 nt552 had no difference between alcoholic and nonalcoholic patients（P＞0.05）. Conclusion: Alcohol can stimulate HBV gene mutations on BCP nt1762 and nt1764. Gene mutations on BCP may enhance the HBV DNA replication and the gene expression, leading to deteriorate the state of the illness.

Key words: Hepatitis B, chronic, Hepatitis, B virus, Hepatitis, alcoholic, Gene mutation, Gene chip

许建民,陈建,王敏,刘福国 . 酒精对慢性乙型肝炎患者乙肝病毒多位点基因突变影响研究[J]. 山东大学学报(医学版), 2006, 44(11): 1135-1138.

XU JIan-min,CHEN Jian,WANG Min,LIU Fu-guo. [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2006, 44(11): 1135-1138.

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