侧脑室注射内吗啡肽-1降低神经病理性疼痛大鼠海马和PAG IL-6mRNA的表达

山东大学学报(医学版)

侧脑室注射内吗啡肽-1降低神经病理性疼痛大鼠海马和PAG IL-6mRNA的表达

孙涛¹,姚尚龙²,,宋文阁¹,傅志俭¹

1. 山东省立医院疼痛科，山东济南 250021；2. 华中科技大学同济医学院附属协和医院麻醉科，湖北武汉 430022

收稿日期:2006-03-29 修回日期:1900-01-01 出版日期:2006-10-24 发布日期:2006-10-24
通讯作者: 孙涛

SUN Tao, SONG Wen-ge, YAO Shang-long,FU Zhi-jian

(1. Department of Pain Management， Shandong Provincial Hospital, Jinan 250021, Shandong, China;2. Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong University

Received:2006-03-29 Revised:1900-01-01 Online:2006-10-24 Published:2006-10-24

摘要/Abstract

摘要： 目的：观察侧脑室微量注射内吗啡肽1（endomorphin1, EM1）对坐骨神经慢性压迫性损伤（chronic constriction injury, CCI）大鼠痛阈以及海马和中脑导水管周围灰质（periaqueductal gray，PAG）中白细胞介素6（interleukin6, IL6）mRNA表达的影响。方法：建立大鼠慢性坐骨神经压迫性损伤模型，侧脑室微量注射EM1(20?μg/20?μl)，手术前1?d和手术后第7d观察其机械性痛敏和热痛敏的变化，术后第7?d将大鼠处死，采用原位杂交观察海马和PAG IL6 mRNA的表达，假手术及侧脑室注射生理盐水作对照。结果：实验发现CCI手术导致大鼠机械性痛阈和热痛阈明显下降，且增加大鼠海马和PAG部位IL6mRNA的表达。侧脑室注射EM1可提高大鼠基础痛阈，且显著改善大鼠神经病理性疼痛状态，降低海马和PAG部位IL6mRNA的表达。结论：侧脑室微量注射EM1可有效抑制神经病理性疼痛，且明显降低海马和PAG IL6的表达。

关键词: 内吗啡肽1, 白细胞介素6 , 慢性压迫性损伤

Abstract: To investigate the effect of intracerebroventricular (i.c.v.) injection of endomorphin1 (EM1) on IL6 mRNA expression level in hippocampus and on PAG in chronic constriction injury (CCI) model of rats. Methods： The CCI model was established and given endomorphin1 (20 μg/20 μl, i.c.v) once daily for 3 consecutive days. The control rats were operated on and injected NS. The mechanical and the thermal pain threshold were measured 1 day before operation and 7 days after operation. The expression level of IL6 mRNA was measured by in situ hybridization. Results： Mechanical allodynia and thermal hyperalgesia were induced in CCI rats. In addition, the expression level of IL6 mRNA in hippocampus and PAG markedly increased. Intracerebroventricular injection of EM1 increased the basic pain threshold of the rats, reversed the CCIinduced existing neuropathic pain state and significantly suppressed the increased expression level of IL6 mRNA induced by CCI. Conclusion： EM1 may be involved in the modulation of neuropathic pain mediated by IL6 in hippocampus and PAG

Key words: Chronic constriction injury, Endormorphin1, Interleukin

孙涛,姚尚龙,宋文阁,傅志俭 . 侧脑室注射内吗啡肽-1降低神经病理性疼痛大鼠海马和PAG IL-6mRNA的表达[J]. 山东大学学报(医学版), 2006, 44(10): 1028-1031.

SUN Tao,SONG Wen-ge,YAO Shang-long,FU Zhi-jian. [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2006, 44(10): 1028-1031.

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