茯苓三种活性成分对秀丽隐杆线虫的体内抗衰老作用

doi:10.6040/j.issn.1671-7554.0.2024.1116

摘要/Abstract

摘要： 目的研究茯苓三种活性成分对秀丽隐杆线虫(Caenorhabditis elegans, C. elegans, N2线虫)寿命和应激能力的影响及机制,初步探究茯苓抗衰老的潜力。方法采用溶剂法提取茯苓中水溶性多糖、酸性多糖和三萜;评估3种活性成分对N2线虫寿命、抗应激、生殖能力和运动能力以及活性氧(reactive oxygen species, ROS)和脂褐素水平的影响;采用实时荧光定量逆转录聚合酶链式反应(quantitative reverse transcription polymerase chain reaction, qRT-PCR)测定衰老相关基因的表达。结果与空白组相比,500 μg/mL水溶性多糖组、62.5 μg/mL酸性多糖组和500 μg/mL茯苓三萜组的N2线虫寿命分别延长12.72%、15.07%和12.55%,并增强其抗氧化应激能力(P<0.05);酸性多糖和三萜能提高N2线虫在热应激和紫外应激状态下的生存率,降低N2线虫体内ROS水平,差异有统计学意义(P<0.05)。3种活性成分可显著降低N2线虫体内脂褐素水平(P<0.05),并不影响其产卵量和运动能力(P>0.05)。茯苓3种活性成分可通过上调daf-16和ctl-2 mRNA及下调daf-2和age-1 mRNA的表达,延长N2线虫寿命。结论茯苓3种活性成分在N2线虫中表现出抗氧化和抗衰老的作用,其机制可能与调控胰岛素/胰岛素样生长因子1信号通路中衰老相关基因的表达有关。

关键词: 茯苓活性成分, 抗衰老, 水溶性多糖, 酸性多糖, 三萜

Abstract: Objective To explore the anti-aging potential of Poria cocos by investigating the effects of its three active ingredients on the life-span and stress ability in Caenorhabditis elegans(C. elegans) and potential mechanisms. Methods Water-soluble polysaccharides, acidic polysaccharides, and triterpenes were extracted from Poria cocos using a solvent extraction method. The effects of these three active ingredients on the lifespan, stress resistance, reproduction ability, and locomotion of C. elegans, as well as their impact on the levels of ROS and lipofuscin, were evaluated. The expression of aging-related genes was determined using qRT-PCR. Results Compared with the control group, water-soluble polysaccharides at 500 μg/mL, acidic polysaccharides at 62.5 μg/mL, and triterpenes from Poria cocos at 500 μg/mL prolonged the lifespan of C. elegans by 12.72%, 15.07% and 12.55%, and enhanced the antioxidant stress resistance(P<0.05), respectively. Acidic polysaccharides and triterpenes increased their survival rates under heat stress and ultraviolet stress and reduced the levels of ROS in the C. elegans, with statistically significant differences(P<0.05). Meanwhile, these three active ingredients significantly reduced lipofuscin levels in C. elegans(P<0.05), and did not affect the egg-laying capacity and motility(P>0.05). The three active ingredients from Poria cocos upregulated the expression of daf-16 and ctl-2 mRNA, while downregulating the expression of daf-2 and age-1 mRNA, thereby extending the lifespan of C. elegans. Conclusion The three active ingredients of Poria cocos exhibited antioxidant and anti-aging effects in C. elegans. The mechanism may be related to regulating the expression of aging-related genes in the insulin/IGF-like signaling pathway.

Key words: Poria cocos active ingredients, Anti-aging, Water-soluble polysaccharides, Acidic polysaccharides, Triterpenes

中图分类号:

R931.6

余玉楠,王平,代君怡,刘军锋,昝俊峰. 茯苓三种活性成分对秀丽隐杆线虫的体内抗衰老作用[J]. 山东大学学报 (医学版), 2025, 63(6): 1-10.

YU Yunan, WANG Ping, DAI Junyi, LIU Junfeng, ZAN Junfeng. In vivo anti-aging effect of poria cocos three active ingredients on caenorhabditis elegans[J]. Journal of Shandong University (Health Sciences), 2025, 63(6): 1-10.

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