山东大学学报 (医学版) ›› 2024, Vol. 62 ›› Issue (5): 79-88.doi: 10.6040/j.issn.1671-7554.0.2024.0134
• 临床医学 • 上一篇
冯绪梅1,宋相庆2,季冠虹2,赵小刚2,肖兆华2
FENG Xumei1, SONG Xiangqing2, JI Guanhong2, ZHAO Xiaogang2, XIAO Zhaohua2
摘要: 目的 探讨血浆外泌体中与食管鳞癌(esophageal squamous cell carcinoma, ESCC)发生相关的microRNAs(miRNAs),并评估其在ESCC诊断中的应用价值。 方法 选取2020年3月至2021年6月山东大学第二医院胸外科收治的55例ESCC患者为ESCC组,另选取55例健康体检者为对照组,提取受试者血浆外泌体,并通过透射电镜、NanoSight粒径分析及Western blotting进行鉴定。筛选阶段,送检对照组、ESCC组术前血浆外泌体RNA各5例进行测序。采用qRT-PCR法对训练集、验证集中差异性miRNAs分子表达进行检测,并通过受试者工作特征(receiver operating characteristic, ROC)曲线评估差异性miRNAs分子的诊断价值。 结果 测序结果显示,与对照组相比,5个miRNAs(miR-548k、miR-6516-5p、let-7b-3p、miR-3934-5p、miR-196a-5p)在ESCC组血浆外泌体中表达显著上调,8个miRNAs(miR-34c-5p、novel_167、 miR-548u、miR-548ah-3p、miR-548p、miR-200a-5p、miR-200b-3p、miR-1228-5p)明显下调。通过训练队列证实,miR-548k在ESCC组血浆外泌体中明显上调(P<0.001),血浆外泌体miR-548k用于ESCC诊断时ROC曲线下面积(area under curve, AUC)为0.918,明显优于miR-34c-5p、miR-548u、miR-6516-5p。通过验证队列进一步证实,miR-548k在ESCC组血浆外泌体中高表达(P<0.001),血浆外泌体miR-548k可用于ESCC患者诊断(AUC值为0.889)。 结论 miR-548k在ESCC组血浆外泌体中表达显著增高,血浆外泌体miR-548k对ESCC诊断有一定的临床应用价值。
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