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山东大学学报 (医学版) ›› 2018, Vol. 56 ›› Issue (12): 19-25.doi: 10.6040/j.issn.1671-7554.0.2018.338

• • 上一篇    

脂肪源性干细胞对内源性皮肤老化的治疗作用

张坤1,2,延冰3,李芳1,2,肖东杰1,2,汪运山1,2,刘华1,2   

  1. 1. 山东大学附属济南市中心医院细胞治疗转化应用研究室, 山东 济南 250013;〓〓〓〓〓〓2. 山东省移植与组织工程技术研究中心, 山东 济南 250013;〓〓〓〓〓〓3. 山东大学附属济南市中心医院胃肠外科, 山东 济南 250013
  • 发布日期:2022-09-27
  • 通讯作者: 刘华. E-mail:liuhuagreen@126.com
  • 基金资助:
    国家自然科学基金青年科学基金(81200950);山东省科技发展计划(2014GSF118173);中国博士后科学基金面上项目(2016M590637);山东省医药卫生科技发展计划(2016WS0139,2016WS0134)

Effect of adipose-derived stem cells on intrinsic skin aging

ZHANG Kun1,2, YAN Bing3, LI Fang1,2, XIAO Dongjie1,2, WANG Yunshan1,2, LIU Hua1,2   

  1. 1. Laboratory of Cell Therapy and Translational Medicine, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China;
    2. Shandong Research Center of Transplantation and Tissue, Jinan 250013, Shandong, China;
    3. Department of Gastrointestinal Surgery, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China
  • Published:2022-09-27

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摘要： 目的 研究脂肪源性干细胞(ADSCs)对内源性皮肤老化的抗衰老作用及机制。 方法 分离培养小鼠皮肤成纤维细胞(MSFCs)及人ADSCs。将MSFCs 分为对照组、模型组、ADSCs条件培养基组。D-半乳糖(D-gal)制备MSFCs衰老模型,ADSCs条件培养基培养。镜下及流式检测ROS、β-半乳糖苷酶活性的变化,RT-PCR 检测caspase3、p53、sirt1基因的表达情况,Western blotting检测Sirt1蛋白表达。 结果 模型组较对照组ROS水平上升(P<0.001)、β-半乳糖苷酶着色增深,而ADSCs条件培养基组较模型组ROS水平降低(P<0.001)、β-半乳糖苷酶着色变浅。RT-PCR检测结果显示模型组较对照组caspase3(P<0.001)、p53(P=0.001)表达升高,sirt1表达下降(P<0.001);而ADSCs条件培养基组较模型组caspase3(P<0.001)、p53(P=0.001)表达下降,sirt1表达升高(P<0.001)。 Western blotting结果显示模型组较对照组Sirt1表达下降(P<0.001),而ADSCs条件培养基组较模型组Sirt1表达升高(P=0.006)。 结论 ADSCs可通过Sirt1通路改善D-gal引起的内源性皮肤老化。

关键词: 脂肪源性干细胞, D-半乳糖, 皮肤老化

Abstract: Objective To observe the effects of adipose-derived stem cells(ADSCs)on intrinsic skin aging, and investigate the underlying mechanisms. Methods Mouse skin fibroblast cells(MSFCs)and human ADSCs were primarily cultured. MSFCs cells were randomly divided into control group, model group, ADSCs conditioned medium(ADSCs-CM)group. MSFCs were treated with D-galactose(D-gal)and ADSCs-CM. The changes of ROS and β-galactosidase were tested by microscopy and flow cytometry, the expressions of caspase3, p53 and sirt1 were detected by RT-PCR, and the expression of Sirt1 protein was detected by Western blotting. Results The level of ROS in model group was higher than that in control group(P<0.001), and the β-galactosidase staining was deepened. The level of ROS in ADSCs-CM group was lower than that in model group(P<0.001), and the β-galactosidase staining was lighter. RT-PCR showed that the expressions of caspase3(P<0.001)and p53(P=0.001)were increased while the expression of sirt1(P<0.001)was decreased in model group compared with those in control group. The expressions of caspase3 山 东 大 学 学 报 (医 学 版)56卷12期 -张坤,等.脂肪源性干细胞对内源性皮肤老化的治疗作用 \=-(P<0.001)and p53(P=0.001)were decreased while the expression of sirt1 (P<0.001)was increased in ADSCs-CM group compared with that in model group. Western blotting showed that the Sirt1 protein expression was decreased in model group compared with that in control group(P<0.001)and increased in ADSCs-CM group compared with that in model group(P=0.006). Conclusion ADSCs show a protective effect on intrinsic skin aging induced by D-gal via Sirt1 signal pathway.

Key words: Adipose-derived stem cells, D-galactose, Skin aging

中图分类号: 

  • R751
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