数字PCR在肿瘤诊疗中的应用进展

doi:10.6040/j.issn.1671-7554.0.2018.1035

摘要/Abstract

摘要： 数字PCR是一种新兴的核酸检测技术,具有灵敏度高、可定量分析的特点。基于数字PCR的液态活检,可用于极微量核酸样本及稀有突变位点的检测,尤其适用于肿瘤治疗过程中需多次反复取样的情况,实现动态监测,为肿瘤诊断、复发提供及时的基因信息。

关键词: 数字PCR, 液态活检, 基因检测, 绝对定量, 肿瘤

Abstract: Digital PCR is a novel technique that facilitates the absolute quantitation of nucleic acid targets with high sensitivity. Digital PCR combined with liquid biopsies can be used in the detection of infinitesimal nucleic acid samples and rare mutations. It is particularly suitable for cancer patients who need repeated sampling. Digital PCR can provide adequate gene information in cancer diagnosis and relapse monitoring.

Key words: Digital PCR, Liquid biopsy, Genetic testing, Absolute quantitation, Cancer

中图分类号:

R73-34

关明,张心菊. 数字PCR在肿瘤诊疗中的应用进展[J]. 山东大学学报 (医学版), 2018, 56(10): 46-50.

GUAN Ming, ZHANG Xinju. Applications of digital PCR in cancer detection and treatment[J]. Journal of Shandong University (Health Sciences), 2018, 56(10): 46-50.

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