安徽省部分地区2015年产小麦真菌污染调查

doi:10.6040/j.issn.1671-7554.0.2016.063

山东大学学报（医学版） ›› 2016, Vol. 54 ›› Issue (9): 92-96.doi: 10.6040/j.issn.1671-7554.0.2016.063

• • 上一篇

安徽省部分地区2015年产小麦真菌污染调查

徐文静¹, 韩小敏²,张靖²,潘琢³,李凤琴²,张立实¹

1. 四川大学华西公共卫生学院营养与食品卫生学系, 四川成都 610041;2. 国家食品安全风险评估中心微生物实验部, 北京 100022;3.石家庄市疾病预防控制中心微生物检验所, 河北石家庄 050010

收稿日期:2016-01-15 出版日期:2016-09-10 发布日期:2016-09-10
通讯作者: 李凤琴. E-mail:lifengqin@cfsa.net.cn; 张立实. E-mail:lishizhang_56@163.com E-mail:lifengqin@cfsa.net.cn; lishizhang_56@163.com
基金资助:
国家食品安全风险评估中心523人才项目;国家自然科学基金青年科学基金(31301489)

Survey on fungi contamination of wheat harvested in 2015 from Anhui Province of China

XU Wenjing¹, HAN Xiaomin², ZHANG Jing², PAN Zhuo³, LI Fengqin², ZHANG Lishi¹

1. Institute of Nutrition and Food Hygiene, West China College of Public Health, Sichuan University, Chengdu 610041, Sichuan, China;
2. Laboratory of Microbiology, China National Center for Food Safety Risk Assessment, Beijing 100022, China;
3. Institute of Microbiology Examination, Shijiazhuang CDC, Shijiazhuang 050010, Hebei, China

Received:2016-01-15 Online:2016-09-10 Published:2016-09-10

摘要/Abstract

摘要： 目的调查安徽省部分地区2015年产小麦真菌污染情况,为评估粮食中真菌侵染及真菌毒素污染状况、进一步开展预测微生物学研究提供依据。方法采集安徽省五地市2015年产未经储存的小麦籽粒,进行感官检查和千粒重计数。采用点种法将麦粒样品接种于含0.1 g/L氯霉素的马铃薯葡萄糖琼脂(PDA)平板,于(28±1 )℃培养5 d后,进行菌落计数和菌种鉴定。结果感官检查显示,每份小麦样品均检出数量不等的赤霉病粒,范围在8.0%~20.0%之间,千粒重平均值范围在39.8 ~48.5 g之间,且赤霉病粒率与千粒重水平呈负相关(r=-0.98)。安徽省五地市小麦样品的真菌污染率为100.0%,且均以交链孢霉为主要污染菌。结论安徽省五地市2015年产小麦赤霉病粒率较高,真菌污染状况较严重,有必要对小麦中的真菌毒素进行检测,并结合我国人群小麦及其制品的消费情况,评估居民通过食用小麦及其制品暴露真菌毒素的风险。

关键词: 赤霉病麦, 真菌, 安徽省, 污染

Abstract: Objective To evaluate the fungi contamination of wheat harvested in 2015 in order to provide the basis for fungi and mycotoxin control and study in predictive microbiology. Methods Organoleptic examinations and thousand-grain weight check were conducted for wheat kernel samples collected without storage from five regions of Anhui Province harvested in 2015. Wheat kernels were sterilized and inoculated onto potato dextrose agar medium plates which contained 0.1 g/L chloramphenicol. The colony-forming units of fungi germinated were enumerated, classified and identified after incubation for 5 d at(28±1)℃. Results Varied amounts of gibberella damaged wheat kernels were observed in each sample, the frequencies ranging from 8.0% to 20.0%. The average value of thousand-grain weight ranged from 39.8 to 48.5 g. The frequency of scabby wheat kernels had negative correlation with thousand-grain weight(r=-0.98). The frequency of wheat samples contaminated by fungi was up to 100.0%, and the predominant was Alternaria species. Conclusion The wheat kernels collected from five regions of Anhui Province harvested in 2015 are seriously contaminated by fungi and the frequency of scabby wheat kernels is pretty high. There is a need to focus on the detection of mycotoxins combined with a risk assessment of different mycotoxins in wheat and its products to Chinese population.

Key words: Scabby wheat, Contamination, Fungi, Anhui Province

中图分类号:

R574

徐文静, 韩小敏,张靖,潘琢,李凤琴,张立实. 安徽省部分地区2015年产小麦真菌污染调查[J]. 山东大学学报（医学版）, 2016, 54(9): 92-96.

XU Wenjing, HAN Xiaomin, ZHANG Jing, PAN Zhuo, LI Fengqin, ZHANG Lishi. Survey on fungi contamination of wheat harvested in 2015 from Anhui Province of China[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(9): 92-96.

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安徽省部分地区2015年产小麦真菌污染调查

Survey on fungi contamination of wheat harvested in 2015 from Anhui Province of China

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