醋酸泼尼松治疗URSA对临床结局及NK细胞表型的影响

doi:10.6040/j.issn.1671-7554.0.2015.839

山东大学学报（医学版） ›› 2016, Vol. 54 ›› Issue (5): 62-65.doi: 10.6040/j.issn.1671-7554.0.2015.839

醋酸泼尼松治疗URSA对临床结局及NK细胞表型的影响

贾新转¹,张娜¹,郭丽娜¹,赵素英¹,董翠¹,魏兰²

1.河北医科大学第四医院生殖医学科, 河北石家庄 050011;2.河北省胸科医院胸外科, 河北石家庄 050041

收稿日期:2015-09-05 出版日期:2016-05-16 发布日期:2016-05-16
通讯作者: 魏兰. E-mail:1134979643@qq.com E-mail:1134979643@qq.com

Effects of prednisone therapy on the clinical outcomes and phenotypes of natural killer cells in patients with URSA

JIA Xinzhuan¹, ZHANG Na¹, GUO Lina¹, ZHAO Suying¹, DONG Cui¹, WEI Lan²

1. Department of Reproductive Medicine, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei, China;
2. Department of Thoracic Surgery, Hebei Provincial Chest Hospital, Shijiazhuang 050041, Hebei, China

Received:2015-09-05 Online:2016-05-16 Published:2016-05-16

摘要/Abstract

摘要： 目的分析醋酸泼尼松治疗原因不明复发性自然流产(URSA)的临床结局(持续妊娠>12周)及治疗前、后NK细胞CD56、CD16变化。方法选择URSA患者160例,分为2组,每组80例。试验组给予醋酸泼尼松及阿司匹林治疗;对照组给予安慰剂及阿司匹林治疗;治疗周期始于孕4~5周止于孕12周,如治疗期间流产即停药。采用流式细胞仪测定治疗前、后外周血NK细胞CD56、CD16百分率。结果试验组持续妊娠>12周百分率高于对照组(P=0.00);试验组治疗后CD16较治疗前降低(P=0.00);对照组治疗前、后CD16差异无统计学意义(P=0.79);试验组、对照组治疗前、后CD56差异无统计学意义(试验组P=0.09,对照组P=0.12)。结论醋酸泼尼松改善URSA患者的临床结局可能与CD16降低、抑制细胞毒性作用有关。

关键词: CD56, CD16, NK细胞, 原因不明复发性自然流产, 醋酸泼尼松

Abstract: Objective To analyze the clinical outcomes(ongoing pregnancy > 12 weeks)and changes of natural killer(NK)cells CD56 and CD16 before and after prednisone treatment in patients with URSA(unexplained recurrent spontaneous abortion, URSA). Methods A total of 160 URSA patients were divided into two groups: experimental group(receiving prednisone and aspirin therapy, n=80), and control group(receiving placebo and aspirin therapy, n=80). The therapy started from 4-5 weeks to 12 weeks of gestation, and stopped if abortion occurred. The percentages of CD56 and CD16 were measured by flow cytometry before and after treatment. Results The percentage of ongoing pregnancy > 12 weeks was significantly higher in the experimental group than in the control group(P=0.00). The level of CD16 markedly dropped after treatment in the experimental group(P=0.00), while it remained unchanged in the control group(P=0.79). There was no statistical difference in the level of CD56 before and after treatment between the two groups(experimental group P=0.09, control group P=0.12). Conclusion Prednisone can improve the clinical outcome in patients with URSA, which may be related to the reduction of CD16 and inhibition of cell toxicity.

Key words: Unexplained recurrent spontaneous abortion, Prednisone, CD56, CD16, Natural Killer cell

中图分类号:

R714.2

贾新转,张娜,郭丽娜,赵素英,董翠,魏兰. 醋酸泼尼松治疗URSA对临床结局及NK细胞表型的影响[J]. 山东大学学报（医学版）, 2016, 54(5): 62-65.

JIA Xinzhuan, ZHANG Na, GUO Lina, ZHAO Suying, DONG Cui, WEI Lan. Effects of prednisone therapy on the clinical outcomes and phenotypes of natural killer cells in patients with URSA[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(5): 62-65.

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醋酸泼尼松治疗URSA对临床结局及NK细胞表型的影响

Effects of prednisone therapy on the clinical outcomes and phenotypes of natural killer cells in patients with URSA

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