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山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (3): 46-49.doi: 10.6040/j.issn.1671-7554.0.2014.951

• 基础医学 • 上一篇    下一篇

金银花提取物对化合物48/80、组胺及P物质诱导小鼠瘙痒模型的作用

白雪1, 黄惠锋2, 管玉东1, 迟连利1,3   

  1. 1. 山东大学国家糖工程技术研究中心, 山东 济南 250100;
    2. 浙江圣兆药物科技股份有限公司, 浙江 杭州 310000;
    3. 山东大学微生物技术国家重点实验室, 山东 济南 250100
  • 收稿日期:2014-12-14 修回日期:2015-01-14 出版日期:2015-03-10 发布日期:2015-03-10
  • 通讯作者: 迟连利, E-mail:lianlichi@sdu.edu.cn E-mail:lianlichi@sdu.edu.cn
  • 基金资助:
    国家自然科学基金(81102783);山东省自然科学基金(ZR2011HQ038)

Effects of Flos Lonicerae Japonicae extract on pruritus induced by compound 48/80, histamine and substance P in ICR mice

BAI Xue1, HUANG Huifeng2, GUAN Yudong1, CHI Lianli1,3   

  1. 1. National Glycoengineering Research Center, Shandong University, Jinan 250100, Shandong, China;
    2. Zhejiang Sandoc Pharmaceutical Science and Technology Co., Ltd. Hangzhou 310000, Zhejiang, China;
    3. State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, Shandong, China
  • Received:2014-12-14 Revised:2015-01-14 Online:2015-03-10 Published:2015-03-10

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摘要： 目的 探讨金银花提取物对化合物48/80、组胺及P物质诱导的小鼠瘙痒模型的作用。方法 采用0.3、0.8、2.4 g/kg 3个不同剂量的金银花提取物将小鼠灌胃给药1 h后, 分别皮内注射致痒物质化合物48/80、组胺和P物质, 随后将小鼠置于观察笼内, 用摄像仪记录1 h内小鼠的行为。结果 金银花提取物可呈剂量依赖性的抑制P物质诱导的小鼠瘙痒, 而对化合物48/80和组胺诱导的小鼠瘙痒没有明显的抑制作用。结论 金银花提取物对P物质诱导的小鼠模型有明显的抗瘙痒作用, 其抗瘙痒活性主要作用部位为角质形成细胞, 而非肥大细胞。

关键词: 金银花, 提取物, 搔痒行为, P物质

Abstract: Objective To study the effect of Flos Lonicerae Japonicae on pruritus induced by various pruritic agents including compound 48/80, histamine and substance P in mice. Methods Flos Lonicerae Japonicae extracts(0.3 g/kg, 0.8 g/kg and 2.4 g/kg) were respectively administered orally to ICR mice 1 h before the injection of compound 48/80, histamine and substance P. Immediately after the injection of pruritogens, the animals were put into the acrylic cage again, and their behaviors were videotaped. Results Flos Lonicerae Japonicae dose-dependently inhibited substance P-induced scratching. However, no inhibitory effect was observed on the scratching behavior induced by histamine or compound 48/80. Conclusion Flos Lonicerae Japonicae shows significant antipruritic effect on substance P-induced pruritus in ICR mice. The primary site of its antipruritic activity is epidermal keratinocytes rather than mast cells.

Key words: Itch-associated response, Extract, Substance P, Flos Lonicerae Japonicae

中图分类号: 

  • R285
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