复合CMC脱细胞真皮基质的抗菌性能

doi:10.6040/j.issn.1671-7554.0.2014.755

摘要/Abstract

摘要： 目的探讨脱细胞真皮基质(ADM)以戊二醛为交联剂复合羧甲基壳聚糖(CMC)后的抗菌性能.方法实验采用2×3析因设计,根据样本状态(湿润、干燥)和分组(复合样片组、单纯样片组、空白对照组)设计实验.应用烧瓶振荡法,利用优化的实验条件,在转速200 r/min、培养温度25 ℃、作用时间1 h的条件下,对复合CMC的ADM进行抗菌性能检测.通过菌落平板计数获得实验前(0 h)、实验后(1 h)的菌落数,计算抑菌率.两组实验中细菌在振摇前后的菌落数差值均符合实验限定标准(差值变化不超过10%).结果湿润状态下,复合样片组对金黄色葡萄球菌的抑菌率为61.17%,对白色念珠菌的抑菌率为39.79%;干燥状态下,复合样片组对金黄色葡萄球菌的抑菌率为0%,对白色念珠菌的抑菌率为15.17%.湿润与干燥两个因素比较,差异有统计学意义(P <0.001).结论复合CMC的ADM这种交联复合物在湿润状态下有一定的抗菌性,干燥状态下不具备抗菌性能,交联效率较低,需进一步改良制备工艺.

关键词: 羧甲基壳聚糖, 抗菌性, 烧瓶振荡法, 抑菌率, 脱细胞真皮基质

Abstract: Objective To explore the antibacterial activity of acellular dermal matrix (ADM) with glutaraldehyde as crosslinking agent composite carboxymethyl chitosan(CMC). Methods The 2×3 factorial design was adopted, and the experiments were designed according to the sample status (wet, dry) and groups (composite sample group, simple sample group, blank control group). Shaking-flask method was used and the optimized experimental conditions (rotational speed 200 r/min, culture temperature 25 ℃, culture time one hour) were used to evaluate the antibacterial activity of acellular dermal matrix composite CMC. To get the bacterial inhibition rate, colony plate count was adopted to obtain the number of bacterial colonies before the experiment(0 h) and after the test(1 h). In the two experimental groups, the number of bacteria accorded with the qualified standard before and after the shaking-flask (change of the number was no more than 10%). Results In wet condition, the antibacterial rate of composite sample group was 61.17% for staphylcoccus aureus, and 39.79% for candida albicans. In dry condition, the antibacterial rate of composite sample group was 0% for staphylcoccus aureus, and 15.17% for candida albicans. There were statistical differences (P <0.001). Conclusion The crosslinking compound has certain antibacterial activity in wet state. In dry condition, it has no antibacterial activity, and the crosslinking efficiency is lower and needs to be improved.

Key words: Shaking-flask method, Carboxymethyl chitosan, Bacterial inhibition rate, Acellular dermal matrix, Antibacterial activity

中图分类号:

R318.8

安祥莲, 郭泾. 复合CMC脱细胞真皮基质的抗菌性能[J]. 山东大学学报（医学版）, 2015, 53(8): 32-37.

AN Xianglian, GUO Jing. Antibacterial activity of acellular dermal matrix composite CMC[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(8): 32-37.

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