2011年济南市某污水河中肠道病毒的分离鉴定与序列分析

doi:10.6040/j.issn.1671-7554.0.2014.418

山东大学学报（医学版） ›› 2014, Vol. 52 ›› Issue (11): 97-100.doi: 10.6040/j.issn.1671-7554.0.2014.418

2011年济南市某污水河中肠道病毒的分离鉴定与序列分析

陶泽新¹, 杨静², 王海岩¹, 林小娟¹, 王素婷¹, 宋立志¹, 徐爱强¹

1. 山东省传染病预防控制重点实验室,山东省疾病预防控制中心, 山东济南 250014;
2. 济宁医学院《中华行为医学与脑科学杂志》编辑部, 山东济宁 272100

收稿日期:2014-06-24 发布日期:2014-11-10
通讯作者: 徐爱强.E-mail:aqxuepi@163.com E-mail:aqxuepi@163.com
基金资助:
国家自然科学基金青年基金(81302481);全球健康-盖茨基金(OPP1039272)

Identification and sequence analysis of human enteroviruses isolated from a polluted river in Jinan City, Shandong Province, 2011

TAO Zexin¹, YANG Jing², WANG Haiyan¹, LIN Xiaojuan¹, WANG Suting¹, SONG Lizhi¹, XU Aiqiang¹

1. Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Jinan 250014, Shandong, China;
2. Jining Medical College, Jining 272100, Shandong, China

Received:2014-06-24 Published:2014-11-10

摘要/Abstract

摘要： 目的监测济南市区地表河水中的人类肠道病毒(HEV),分析其基因特征.方法于2011年5、6、7月份对流经济南市中心城区的西泺河各采集1份河水标本,经过阴离子膜吸附的方法浓缩后,接种RD、HEp-2和L20b细胞进行病毒分离.测定分离株VP1编码区序列并进行序列分析.结果共分离到HEV 9株,其中埃可病毒6型(Echovirus 6,E6)4株,柯萨奇B2病毒(Coxsackievirus B2,CVB2)5株.VP1核苷酸序列分析显示,4株E6病毒与2010年山东省临沂市脑膜炎病例分离株的同源性较高(95.9%~96.7%),提示当地有发生E6引起的脑膜炎病例的可能性;与济南市2010年污水分离株的同源性为94.5%~99.7%.5株CVB2病毒之间有较高的核苷酸同源性(98.4%~100%),与往年山东省急性弛缓性麻痹病例分离株之间同源性为80.9%~95.2%.结论生活污水的排入河可作为HEV环境监测的采样点,我国无专门HEV病例监测系统,环境监测是了解一个地区HEV流行的重要途径.

关键词: 柯萨奇病毒感染, 肠道病毒属, 环境监测, 埃可病毒6型,人, 种系发生

Abstract: Objective To investigate the human enteroviruses (HEVs) in Xiluo River which flows through the metropolitan area of Jinan City, and to analyze the genetic characterization of the isolates. Methods Water samples were collected monthly from the river during May to July, 2011. The specimens were concentrated by anionic membrane absorption and elution, and inoculated on RD, HEp-2, and L20b cell lines. The VP1 coding regions of the isolates were sequenced and phylogeneticly analyzed. Results Altogether 9 HEVs, 4 echovirus 6 (E6) and 5 coxsackievirus B2 (CVB2), were isolated. VP1 sequence analysis revealed the river E6 had high homology (95.9%-96.7%) with the aseptic meningitis isolate found in Linyi City in 2010, suggesting the local occurrence of E6 was associated with aseptic meningitis. River E6 also had high homology (94.5%-99.7%) with prevalent E6 strains in Jinan City in 2010. The 5 CVB2 strains were highly homologous (98.4%-100%), which had 80.9%-95.2% homology with isolates from previous acute flaccid paralysis cases in Shandong Province. Conclusion The domestic sewage-polluted river can serve as sampling site for HEV environmental surveillance, which offers an effective approach to comprehend the local HEV circulation in the context of no specialized surveillance system in China.

Key words: Enterovirus, Environmental monitoring, Coxsackievirus infections, Phylogeny, Echovirus 6, human

中图分类号:

R373

陶泽新, 杨静, 王海岩, 林小娟, 王素婷, 宋立志, 徐爱强. 2011年济南市某污水河中肠道病毒的分离鉴定与序列分析[J]. 山东大学学报（医学版）, 2014, 52(11): 97-100.

TAO Zexin, YANG Jing, WANG Haiyan, LIN Xiaojuan, WANG Suting, SONG Lizhi, XU Aiqiang. Identification and sequence analysis of human enteroviruses isolated from a polluted river in Jinan City, Shandong Province, 2011[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(11): 97-100.

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2011年济南市某污水河中肠道病毒的分离鉴定与序列分析

Identification and sequence analysis of human enteroviruses isolated from a polluted river in Jinan City, Shandong Province, 2011

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